Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Common transcriptomic coding gene signature in the brain and the blood after focal brain ischemia in monkey as a tool to study neuroprotection

ABSTRACT: Common transcriptomic coding gene signature in the brain and the blood after focal brain ischemia in monkey as a tool to study neuroprotection

PROVIDER: PRJNA420096 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Common transcriptomic coding gene signature in the brain and the blood after focal brain ischemia in monkey as a tool to study neuroprotection

Project description:Common transcriptomic coding gene signature in the brain and the blood after focal brain ischemia in monkey as a tool to study neuroprotection

2019-10-08 | GSE107452 | GEO

Transcriptome of amniotic fluid in vervet monkey

Project description:Transcriptomic profiling is a crucial tool for understanding growth, development, behavior and predisposition to diseases and the development of health biomarkers. Here, we demonstrate the feasibility of transcriptomic assessment of cell-free fetal RNA in vervet monkey amniotic fluid supernatant (AFS).

2019-09-13 | GSE119908 | GEO

AKT1E17K activates focal adhesion kinase and promotes melanoma brain metastasis

Project description:AKT1E17K activates focal adhesion kinase and promotes melanoma brain metastasis

| PRJNA507777 | ENA

Long Oligonucleotide Microarrays for African Green Monkey Gene Expression Profile Analysis

Project description:Nonhuman primates including African Green Monkey (AGM) are important models for biomedical research. The information on monkey genomes is still limited and no versatile gene expression screening tool is available. We tested human whole genome microarrays for cross-species reactivity with AGM transcripts using both long oligonucleotide arrays (60-mer probes, Applied Biosystems-ABI) and short oligonucleotide arrays (25-mer, Affymetrix). Using the long oligonucleotide arrays we detected four times more AGM transcripts than with the short oligonucleotide technology. The number of detected transcripts was comparable to that detected using human RNA, with 87% of the detected genes being shared between both species. The specificity of the signals obtained with the long oligonucleotide arrays was determined by analyzing the transcriptome of concanavalin A activated CD4+ T cells versus non-activated T cells of two monkey species AGM and macaque. For both species, the genes showing the most significant changes in expression, such as IL-2R, were those known to be also regulated in human CD4+ T cell activation. Finally, tissue-specificity of the signals was established by comparing the transcription profiles of AGM brain and tonsil cells. In conclusion, the ABI human microarray platform provides a highly valuable tool for the assessment of AGM gene expression profiles. Keywords: Defining a microarray platform to study the AGM transcriptome by cross-species and cross-platform comparison

2007-10-01 | GSE6982 | GEO

Targeted activation of CREB in reactive astrocytes is neuroprotective in focal acute cortical injury.

Project description:Transcriptome profiling of mice overexpressing a constitutively active form of CREB in astrocytes (VP16-CREB) vs WT mice, both in normal conditions and after a focal cryolesion (C) in the parietal cortex. Goal is to determine which astrocytic genes are responsible for the neuroprotection we observed in VP16-CREB mice after injury.

2016-04-27 | GSE68187 | GEO

Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain - Study 2

Project description:Monkeys were treated with a single i.v 200 mg/kg dose of hPara.09 or Anti-gD (Isotype control). Brain was harvested on days 4 and 28, and RNA was extracted from the cerebral cortex. The following groups are included: Group 1: Day 4 gD 200mg/kg n=6; Group 2: Day 4 hPara.09 200 mg/kg n=6; Group 3: Day 28 gD 200mg/kg n= 6; Group 4: Day 28 hPara.09 200 mg/kg n=6 (24 animals total). To assess expression heterogeneity between brain samples, 2 different frontal cortex punches were taken from each monkey, resulting in 48 total samples.

2024-12-05 | GSE246092 | GEO

Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain

Project description:Transcriptional Responses to TREM2 Agonist Antibody Treatment in Cynomologus Monkey Brain

| PRJNA1006736 | ENA

Neuroprotection in diet-induced ketotic rat brain after focal ischemia.

Project description:Neuroprotective properties of ketosis may be related to the upregulation of hypoxia inducible factor (HIF)-1alpha, a primary constituent associated with hypoxic angiogenesis and a regulator of neuroprotective responses. The rationale that the utilization of ketones by the brain results in elevation of intracellular succinate, a known inhibitor of prolyl hydroxylase (the enzyme responsible for the degradation of HIF-1alpha) was deemed as a potential mechanism of ketosis on the upregulation of HIF-1alpha. The neuroprotective effect of diet-induced ketosis (3 weeks of feeding a ketogenic diet), as pretreatment, on infarct volume, after reversible middle cerebral artery occlusion (MCAO), and the upregulation of HIF-1alpha were investigated. The effect of beta-hydroxybutyrate (BHB), as a pretreatment, via intraventricular infusion (4 days of infusion before stroke) was also investigated following MCAO. Levels of HIF-1alpha and Bcl-2 (anti-apoptotic protein) proteins and succinate content were measured. A 55% or 70% reduction in infarct volume was observed with BHB infusion or diet-induced ketosis, respectively. The levels of HIF-1alpha and Bcl-2 proteins increased threefold with diet-induced ketosis; BHB infusions also resulted in increases in these proteins. As hypothesized, succinate content increased by 55% with diet-induced ketosis and fourfold with BHB infusion. In conclusion, the biochemical link between ketosis and the stabilization of HIF-1alpha is through the elevation of succinate, and both HIF-1alpha stabilization and Bcl-2 upregulation play a role in ketone-induced neuroprotection in the brain.

| S-EPMC3621146 | biostudies-literature

A novel class of early responder T cells are indispensable for neuroprotection and functional recovery after ischemic stroke

Project description:We report that CD8+CD122+CD49dlow regulatory-like T cells (CD8+TRLs) are among the earliest lymphocytes to infiltrate mouse brain after ischemic stroke, temper inflammation, and confer neuroprotection.

2022-08-03 | GSE201054 | GEO

Neuron-Derived Estrogen is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain

Project description:Neuron-Derived Estrogen is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain

| PRJNA601790 | ENA