Maintenance of human hematopoietic stem cells in vitro using chemical cocktails
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA420484 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6333830_1.fastq.gz | Fastqsanger.gz | |||
| SRR6333830_2.fastq.gz | Fastqsanger.gz | |||
| SRR6333831_1.fastq.gz | Fastqsanger.gz | |||
| SRR6333831_2.fastq.gz | Fastqsanger.gz | |||
| SRR6333832_1.fastq.gz | Fastqsanger.gz |
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2015-04-27 | E-GEOD-68311 | biostudies-arrayexpress
Project description:Neural progenitor cells (NPCs) can be induced from somatic cells by defined factors. Here we report that NPCs can be generated from mouse embryonic fibroblasts by a chemical cocktail, namely VCR (V, VPA, an inhibitor of HDACs; C, CHIR99021, an inhibitor of GSK-3 kinases and R, Repsox, an inhibitor of TGF-β pathways), under a physiological hypoxic condition. These chemical-induced NPCs (ciNPCs) resemble mouse brain-derived NPCs regarding their proliferative and self-renewing abilities, gene expression profiles, and multipotency for different neuroectodermal lineages in vitro and in vivo. Further experiments reveal that alternative cocktails with inhibitors of histone deacetylation, glycogen synthase kinase, and TGF-β pathways show similar efficacies for ciNPC induction. Moreover, ciNPCs can also be induced from mouse tail-tip fibroblasts and human urinary cells with the same chemical cocktail VCR. Thus our study demonstrates that lineage-specific conversion of somatic cells to NPCs could be achieved by chemical cocktails without introducing exogenous factors.
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Project description:Single cell RNA-seq is a powerful tool for studying complex biological systems such as HSPCs cultured in vitro. We detected 11, 3, 4, 8 and 8 subpopulations in uncultured CD34+ cells,control cells, CD34+ cells with the treatment of CFO, cytokines and CFO + cytokines using t-Distributed Stochastic Neighbor Embedding (t-SNE) analysis.
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