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Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma

ABSTRACT: Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma

PROVIDER: PRJNA420852 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6339020.fastq.gz	Fastqsanger.gz
	SRR6339030.fastq.gz	Fastqsanger.gz
	SRR6339030_1.fastq.gz	Fastqsanger.gz
	SRR6339030_2.fastq.gz	Fastqsanger.gz
	SRR6339021.fastq.gz	Fastqsanger.gz

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Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma [RRBS]

Project description:PD-L1 is a ligand for the inhibitory PD1 receptor on T cells and its expression in some cancers inhibits anti-cancer immune response. In melanoma, PD-L1 expression is induced in response to immune stimuli but in a proportion of melanomas it is constitutively expressed. Factors that drive constitutive expression of PD-L1 are unknown. Here we performed genome-scale methylation analysis of six cell lines that constitutively express PD-L1 (PD-L1 positive, referred to as PD-L1CON) and six cell lines that only express PD-L1 after treatment with IFN- (PD-L1 negative, referred to as PD-L1IND)

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Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma [RNA-Seq]

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Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma [RRBS]

Project description:Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma [RRBS]

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Project description:Blocking the PD-1/PD-L1 immunosuppressive pathway has shown promise in the treatment of certain cancers including melanoma. This study investigates differences in the gene expression profiles of human melanomas that do or do not display the immunosuppressive protein PD-L1. Further understanding of genes expressed within the tumor microenvironment of PD-L1+ tumors may lead to improved rationally designed treatments. Gene expression profiling was performed on total RNA extracted by laser capture microdissection from 11 archived formalin-fixed paraffin-embedded (FFPE) melanoma specimens, 5 of which were PD-L1 positive and 6 PD-L1 negative. Details of the design, and the gene signatures found are given in the paper associated with this GEO Series: Janis M. Taube, Geoffrey D. Young, Tracee L. McMiller, Shuming Chen, January T. Salas, Theresa S. Pritchard, Haiying Xu, Alan K. Meeker, Jinshui Fan, Chris Cheadle, Alan E. Berger, Drew M. Pardoll, and Suzanne L. Topalian, Differential expression of immune-regulatory genes associated with PD-L1 display in melanoma: implications for PD-1 pathway blockade, Clin Cancer Res 2015, in press.

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Global hypomethylation mediated changes regulate constitutive PD-L1 expression in melanoma

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