A Major Chromatin Regulator Determines Resistance of Tumor Cells to T cell Mediated Killing
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA420997 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6344930.fastq.gz | Fastqsanger.gz | |||
| SRR6344940.fastq.gz | Fastqsanger.gz | |||
| SRR6344950.fastq.gz | Fastqsanger.gz | |||
| SRR6344931.fastq.gz | Fastqsanger.gz | |||
| SRR6344941.fastq.gz | Fastqsanger.gz |
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Project description:A major chromatin regulator determines resistance of tumor cells to T cell–mediated killing
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Project description:Many human cancers are resistant to immunotherapy, for reasons that are poorly understood. We used a genome-scale CRISPR-Cas9 screen to identify mechanisms of tumor cell resistance to killing by cytotoxic T cells, the central effectors of antitumor immunity. Inactivation of >100 genes-including Pbrm1, Arid2, and Brd7, which encode components of the PBAF form of the SWI/SNF chromatin remodeling complex-sensitized mouse B16F10 melanoma cells to killing by T cells. Loss of PBAF function increased tumor cell sensitivity to interferon-γ, resulting in enhanced secretion of chemokines that recruit effector T cells. Treatment-resistant tumors became responsive to immunotherapy when Pbrm1 was inactivated. In many human cancers, expression of PBRM1 and ARID2 inversely correlated with expression of T cell cytotoxicity genes, and Pbrm1-deficient murine melanomas were more strongly infiltrated by cytotoxic T cells.
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