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The SWR1 Complex Regulates Active DNA Demethylation in Arabidopsis

ABSTRACT: The SWR1 Complex Regulates Active DNA Demethylation in Arabidopsis

PROVIDER: PRJNA421283 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6350650_1.fastq.gz	Fastqsanger.gz
	SRR6350650_2.fastq.gz	Fastqsanger.gz
	SRR6350660_1.fastq.gz	Fastqsanger.gz
	SRR6350660_2.fastq.gz	Fastqsanger.gz
	SRR6350651_1.fastq.gz	Fastqsanger.gz

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Histone acetylation recruits the SWR1 complex to regulate active DNA demethylation in Arabidopsis

Project description:The eukaryotic genome is divided into regions of heterochromatin and euchromatin. The histone variant H2A.Z specifically localizes at euchromatin and displays a genome-wide anti-correlation with DNA methylation. DNA methylation plays a central role in the epigenetic regulation of many eukaryotic genomes. Active DNA demethylation is critical for controlling the epigenome in plants and mammals. Yet, little is known about how DNA demethylases are recruited to target loci. Here we report that SWR1, a conserved histone H2A.Z deposition complex, regulates DNA demethylation in Arabidopsis thaliana by recruiting the plant DNA demethylase ROS1. A forward genetic screen for anti-silencing mutants identified two SWR1 components, PIE1 and ARP6, as cellular factors required for ROS1-mediated DNA demethylation. We further discovered two bromodomain-containing proteins, the methyl-DNA-binding protein AtMBD9, and NPX1, a plant homolog of ScBDF1 in yeast, as components of the SWR1 complex in Arabidopsis. AtMBD9 and NPX1 function redundantly in preventing DNA hypermethylation and transcriptional gene silencing by recognizing histone acetylation marks established by IDM1, a known regulator of DNA demethylation. We show that IDM1 is required for H2A.Z deposition in many genomic regions targeted for active DNA demethylation. We found that H2A.Z interacts with ROS1 and is required for locus-specific DNA demethylation and antisilencing. Our results reveal a role of H2A.Z in active DNA demethylation, and a mechanism through which DNA demethylases can be recruited to target regions by specific histone acetylation marks.

2019-08-01 | GSE115170 | GEO

Histone acetylation recruits the SWR1 complex to regulate active DNA demethylation in Arabidopsis

Project description:Histone acetylation recruits the SWR1 complex to regulate active DNA demethylation in Arabidopsis

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Active DNA demethylation regulates MAMP-triggered immunity in Arabidopsis

Project description:Active DNA demethylation regulates MAMP-triggered immunity in Arabidopsis

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Active DNA Demethylation Regulates Plant Gamete and Zygote Genome Reprogramming

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The SWR1 Complex Regulates Active DNA Demethylation in Arabidopsis

Project description:Using BS-Seq to provide single-base resolution of DNA methylation status in 35S-SUC2 WT and antisilencing mutants (arp6, pie1, h2a.z, idm1, and ros1)

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Polycomb Repressive Complex 1 regulates transcription of active genes

Project description:Polycomb Repressive Complex 1 regulates transcription of active genes

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A novel BRAT1–BRP1 complex recognizes histone acetylation and mediates active DNA demethylation and transcriptional activation in Arabidopsis [BS-Seq]

Project description:Active DNA demethylation is an important epigenetic phenomenon in many eukaryotes. In Arabidopsis thaliana, ROS1, a 5-methylcytosine DNA glycosylase, is responsible for active DNA demethylation via a base excision repair process. Here, we found that Bromodomain and ATPase domain-containing protein 1 (BRAT1) associates with BRP1 (BRAT1 Partner 1) and forms a tight BRAT1–BRP1 complex required for DNA demethylation. To identify hypermethylated loci at the whole-genome level in brat1, brp1, and ros1, we performed whole-genome bisulfite sequencing. Compare the DNA methylation profiles of 10-day old seedlings materials of mutants (brat1, brp1, and ros1) to wild type by whole-genome bisulfite sequencing.

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NuA4 and SWR1 complex in Plasmodium falciparum

Project description:In this study, we identified an MYST-associated complex in P. falciparum, which contains 11 of 13 conserved NuA4 subunits based on their domain structures. Meanwhile, reciprocal pulldowns not only confirmed the NuA4-like complex but also identified an SWR1-like complex, a histone remodeling complex, in P. falciparum.

2024-03-18 | PXD046547 | Pride

A novel BRAT1–BRP1 complex recognizes histone acetylation and mediates active DNA demethylation and transcriptional activation in Arabidopsis [BS-Seq]

2015-04-14 | GSE67798 | GEO

In the absence of H2A.Z, the SWR1 histone replacement complex causes genetic instability, stress and genome transcription misregulation

Project description:The SWR1 complex replaces the canonical histone H2A with the variant H2A.Z (Htz1 in yeast) at specific chromatin regions. This dynamic alteration in nucleosome structure provides a molecular mechanism to regulate transcription. Here we analysed the transcription profiles of single and double mutants and wild-type cells by whole-genome microarray analysis. Our results indicate that genome-wide transcriptional misregulation in htz1∆ can be partially or totally suppressed if SWR1 is not formed (swr1∆), if it forms but cannot bind to chromatin (swc2∆), or if it binds to chromatin but has no histone replacement activity (swc5∆). These results suggest that in htz1∆ the nucleosome remodelling activity of SWR1 affects chromatin integrity because of an attempt to replace H2A with Htz1 in the absence of the latter. Three biological independent replicates were use for each strain

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