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Genome-Wide Identification of ABA Responsive microRNAs in the root and stem of Populus euphratica

ABSTRACT: Genome-Wide Identification of ABA Responsive microRNAs in the root and stem of Populus euphratica

PROVIDER: PRJNA421492 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6356670.fastq.gz	Fastqsanger.gz
	SRR6359100.fastq.gz	Fastqsanger.gz
	SRR6356671.fastq.gz	Fastqsanger.gz
	SRR6359101.fastq.gz	Fastqsanger.gz
	SRR6356672.fastq.gz	Fastqsanger.gz

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Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

Project description:Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

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Genome-Wide Identification of ABA Responsive microRNAs in the stem of Populus euphratica

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A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica

Project description:We present an efficient method to genome-wide discover new and drought stress responsive miRNAs in P. euphratica. High throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 189 new miRNAs which belonged to 120 families were identified, a large increasing to the number of P. euphratica miRNAs. Target prediction and degradome sequencing verification of 22 new and 21 conserved miRNA targets showed these targets were involved in multiple biological processes, including transcription regulation and response to stimulus. Furthermore, comparison of high-throughput sequencing with miRNA microarray profiling data indicated that 104 miRNA sequences were up-regulated, while 27 were down-regulated under drought stress. This preliminary characterization based on our findings provided a framework for future analysis of miRNA genes and their roles in key traits of poplar as stress resistance plant breeding and environment protection usage.

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A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica

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Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

Project description:More then 10 million raw reads were acquired. In total, 238 unique mature sequences which contain 142 conserved miRNA and 96 novel miRNAs were identified.

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Genome-Wide Identification of ABA Responsive microRNAs in the root and stem of Populus euphratica

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-01 | GSE107823 | GEO

ABA-responsive gene expression under shade

Project description:ABA-responsive gene expression under shade

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Project description:A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica

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Genome-Wide Identification of ABA Responsive microRNAs in the stem of Populus euphratica

Project description:More then 10 million raw reads were acquiredIn total, 247 unique mature sequences which contain 149 conserved miRNA and 98 novel miRNAs were identified.

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iSB09 elicits ABA-responsive gene expression in Solanum lycopersicum seedlings

Project description:To compare the genome-wide transcriptional effect of ABA and iSB09 in tomato plants, we performed RNA-seq analysis of mock-, 10 uM ABA- or 20 uM iSB09-treated plants. Differential gene expression analysis between mock- and ABA-treated or iSB09-treated seedlings was done with DESeq2 and genes with an absolute value of log2 fold change (log2FC) > 1 or (log2FC) < -1 and p-adjusted value (padj) < 0.05 were selected. iSB09 upregulated and downregulated genes represent a subset of the ABA-responsive genes, which reflects the activation of PYL1-like and PYL4-like ABA receptors in tomato seedlings.

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