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Decoding microglia responses to psychosocial stress reveals blood-brain barrier breakdown that may drive stress susceptibility

ABSTRACT: Decoding microglia responses to psychosocial stress reveals blood-brain barrier breakdown that may drive stress susceptibility

PROVIDER: PRJNA421967 | ENA |

REPOSITORIES: ENA

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Decoding microglia responses to psychosocial stress reveals blood-brain barrier breakdown that may drive stress susceptibility

Project description:An animal’s ability to cope with or succumb to deleterious effects of chronic psychological stress may be rooted in the brain’s immune responses manifested in microglial activity. Mice subjected to chronic social defeat (CSD) were categorized as susceptible (CSD-S) or resilient (CSD-R) based on behavioral phenotyping, and their microglial RNAs were isolated and analyzed by global gene expression microarrays. Microglia transcriptome from CSD-S mice was enriched for pathways that describe phases of CNS healing to sterile injury including, inflammation, oxidative stress, debris clearance, and wound resolution. Histochemical experiments confirmed the array predictions: CSD-S microglia showed elevated phagocytosis and oxidative stress, and the brains of CSD-S but not CSD-R or HC mice showed vascular leakage of intravenously injected fluorescent tracers. The results suggest that the inflammatory profile of CSD-S microglia may be precipitated by leakage of blood-born substances into brain parenchyma. We hypothesize that these CNS-centric responses contribute to the stress-susceptible behavioral phenotype.

2018-07-31 | GSE107941 | GEO

Decoding microglia responses to psychosocial stress reveals blood-brain barrier breakdown that may drive stress susceptibility.

Project description:An animal's ability to cope with or succumb to deleterious effects of chronic psychological stress may be rooted in the brain's immune responses manifested in microglial activity. Mice subjected to chronic social defeat (CSD) were categorized as susceptible (CSD-S) or resilient (CSD-R) based on behavioral phenotyping, and their microglia were isolated and analyzed by microarray. Microglia transcriptomes from CSD-S mice were enriched for pathways associated with inflammation, phagocytosis, oxidative stress, and extracellular matrix remodeling. Histochemical experiments confirmed the array predictions: CSD-S microglia showed elevated phagocytosis and oxidative stress, and the brains of CSD-S but not CSD-R or non-stressed control mice showed vascular leakage of intravenously injected fluorescent tracers. The results suggest that the inflammatory profile of CSD-S microglia may be precipitated by extracellular matrix degradation, oxidative stress, microbleeds, and entry and phagocytosis of blood-borne substances into brain parenchyma. We hypothesize that these CNS-centric responses contribute to the stress-susceptible behavioral phenotype.

| S-EPMC6062609 | biostudies-literature

GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency

Project description:GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency

| PRJNA857449 | ENA

Neurobehavioral abnormalities following prenatal psychosocial stress are differentially modulated by maternal environment

Project description:The goal of this study was to compare transcriptional profiles of the amygdala from control and (PS) prenatally stressed male and female offspring to elucidate sex-specific molecular mechanisms underlying behavioral and neuroendocrine abnormalities observed after in-utero psychosocial stress exposure. Amygdalar mRNA profiles of 28 day old control and PS male and female mice were generated by preparing sequencing libraries using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina. After indexing, enrichment through 8 cycles of PCR, and passing initial quality control metrics, individually indexed and compatible libraries were proportionally pooled and sequenced using Nextseq 550 sequencer. The sequencing setting of single read 1 × 85 bp to generate ∼50 M reads per sample was used. Differentially expressed genes (DEGs) were analyzed using RUVseq and EdgeR. Results were validated via qPCR We find psychosocial PS results in the emergence of anxiety-like behaviors, an altered behavioral coping strategy to stress, and anhedonia in male and female offspring. Neuroendocrine abnormalities, evidenced by acute stress-induced HPA axis hyperactivity, are only observed in PS female offspring. Our RNA-seq analysis reveals these phenotypic changes in PS offspring to be associated with sex-specific disturbances in genes associated with synaptic transmission, including genes associated with glutamatergic and GABAergic signaling in PS males, and upregulation of DBH, known to regulate NE synthesis, in PS females. Our study represents one of the first detailed analysis of amygdalar transcriptomes generated by RNA-seq comparing control vs prenatally stressed offspring, identifying sex-specific changes in response to in-utero psychosocial stress exposure.

2022-02-08 | GSE189330 | GEO

Psychosocial stress reflected by the liver transcriptome in the pig

Project description:Unfamiliar individuals (German Landrace pigs) with different aggressiveness were mixed. Subsequently hepatic transcriptome profiles were analysed at postnatal stage (190 dpn) per psychosocial group 6 pigs were collected for expression profiling

2013-12-31 | E-GEOD-49290 | biostudies-arrayexpress

Psychosocial stress reflected by the PBMC transcriptome in the pig

Project description:Unfamiliar individuals (German Landrace pigs) with different aggressiveness were mixed. Subsequently PBMC transcriptome profiles were analysed at postnatal stage (190 dpn) Per psychosocial group 7-8 pigs were collected for expression profiling

2013-12-31 | E-GEOD-44992 | biostudies-arrayexpress

Transcriptomic responses to psychosocial stress in vervet monkey (Chlorocebus sabaeus)

Project description:Transcriptomic responses to psychosocial stress in vervet monkey (Chlorocebus sabaeus)

| PRJNA494868 | ENA

Transcriptomic responses to psychosocial stress in vervet monkey (Chlorocebus sabaeus)

Project description:To identify genes involved in responses to psychosocial stressor, we analysed RNAseq transcriptomic profiles in whole blood of 15 juvenile male vervet monkeys from the Caribbean island of St. Kitts at three experimental time points: day 0 (baseline), day 3 and day 5 of exposure to the stressor.

2019-12-12 | GSE120900 | GEO

Psychosocial stress-induced brain atrophy and its suppression via theanine intake

Project description:Psychosocial stress-induced brain atrophy and its suppression via theanine intake

| PRJNA493551 | ENA

GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency

Project description:We performed high-throughput profiling of gene expression in the anterior cingulate cortex of stress-vulnerable BALB/c mice subjected to social defeat stress. BALB/c mice were subjected to 5-day of social defeat stress and euthanized for tissue collection. We then conducted the transcriptomics analysis. Our study provided insights into the understanding of the molecular mechanisms underlying the behavioral response to stress.

2023-01-31 | GSE207854 | GEO

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