Project description:We developed a novel method for RNA-seq in Trypanosomatids called ‘Spliced-Leader Sequencing’ (SL-seq). The method is based on the fact that the 5’ end of Trypanosomatid mRNA is starts with a SL-sequence, and specifically enriches SL-containing RNA prior to sequencing. In this study we compared the performance and functional results obtained with SL-seq to those generated with conventional Illumina Stranded mRNA prep. Therefore we sequenced mRNA of Leishmania donovani logarithmic phase promastigotes, stationary phase promastigotes and intracellular amastigotes with both methods. We also sequenced controlled dilutions of Leishmania RNA with human RNA.
Project description:To further development of our gene expression, we have employed whole genome microarray expression profiling. Human cord blood CD34+ cells from healthy donors was cultured for 2 days with or without SL-13R.
Project description:RNA was isolated from LNCaP-SL after 24 hours of circulating small extracellular vesicles (csEV) single dose treatment (1×10^10 particles/mL).
Project description:Oryza sativa Japonica Group sl, Similar to Cytochrome P450 71A1 (EC 1.14.-.-) (CYPLXXIA1) (ARP-2), is differentially expressed in 28 experiment(s);
Project description:Oryza sativa Japonica Group sl, Similar to Cytochrome P450 71A1 (EC 1.14.-.-) (CYPLXXIA1) (ARP-2), is expressed in 8 baseline experiment(s);
Project description:Although previous research has established links between strigolactone (SL) and drought, in this study, we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. We have employed a comprehensive approach integrating transcriptome, proteome, phytohormone analyses, and physiological data to unravel differences between wild-type and hvd14 plants when responding to drought. Here we deposited control samples, tissue collected at 25DAS (Days after sowing)