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Groundwater metagenome

ABSTRACT: 13C Plant polymer SIP (Hainich CZE H41)

PROVIDER: PRJNA422383 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6376230_1.fastq.gz	Fastqsanger.gz
	SRR6376230_2.fastq.gz	Fastqsanger.gz
	SRR6376231_1.fastq.gz	Fastqsanger.gz
	SRR6376231_2.fastq.gz	Fastqsanger.gz
	SRR6376232_1.fastq.gz	Fastqsanger.gz

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Project description:We demonstrate the feasibility of total RNA-SIP in experiments where microbes from a hydrocarbon-contaminated aquifer were studied in microcosms with 13C-labelled-toluene to understand their adaptation to the simultaneous availability of low levels of different electron acceptors. SIP successfully resolved the involvement of microaerobic vs. aerobic and anaerobic populations. Under microoxic, nitrate-amended conditions hydrocarbon degradation was actually stimulated, but transcripts of denitrification showed no signs of 13C-labelling. The expression of distinct oxygenase-based catabolic pathways for toluene degradation was clearly apparent in 13C-labelled mRNA. We discuss how these direct insights into the gene expression and adaptation mechanisms within complex degrader communities can guide more integrated approaches in monitoring and restoration of contaminated sites.

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