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Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions [scRNA-seq]

ABSTRACT: Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions [scRNA-seq]

PROVIDER: PRJNA422884 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6386700.fastq.gz	Fastqsanger.gz
	SRR6386710.fastq.gz	Fastqsanger.gz
	SRR6386720.fastq.gz	Fastqsanger.gz
	SRR6386730.fastq.gz	Fastqsanger.gz
	SRR6386740.fastq.gz	Fastqsanger.gz

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Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions

Project description:Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions

| PRJNA422886 | ENA

Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions [bulk RNA-seq]

Project description:Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions [bulk RNA-seq]

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Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions [scRNA-seq]

Project description:Neural crest cells migrate throughout the embryo, but how cells move in a directed and collective manner has remained unclear. Here, we perform the first single-cell transcriptome analysis of cranial neural crest cell migration at three progressive stages in chick and identify and establish hierarchical relationships between cell position and time-specific transcriptional signatures. We determine a novel transcriptional signature of the most invasive neural crest Trailblazer cells that is consistent during migration and enriched for approximately 900 genes. Knockdown of several Trailblazer genes shows significant but modest changes to total distance migrated. However, in vivo expression analysis by RNAscope and immunohistochemistry reveals some salt and pepper patterns that include strong individual Trailblazer gene expression in cells within other subregions of the migratory stream. These data provide new insights into the molecular diversity and dynamics within a neural crest cell migratory stream that underlie complex directed and collective cell behaviors.

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Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions [bulk RNA-seq]

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DAN (NBL1) promotes collective neural crest migration by restraining uncontrolled invasion

Project description:Neural crest cells are both highly migratory and significant to vertebrate organogenesis. However, the signals that regulate neural crest cell migration remain unclear. Here, we test the function of DAN, a BMP antagonist we detected by analysis of chick cranial mesoderm. Our analysis shows that, prior to neural crest cell exit from the hindbrain, DAN is expressed in the mesoderm, then it becomes absent along cell migratory pathways. Cranial neural crest and metastatic melanoma cells avoid DAN protein stripes in vitro. Addition of DAN reduces the speed of migrating cells, in vivo and in vitro respectively. In vivo loss-of-function of DAN results in enhanced neural crest cell migration by increasing speed and directionality. Computer model simulations support the hypothesis that DAN restrains cell migration by regulating cell speed. Taken together, our results identify DAN as a novel factor that inhibits uncontrolled neural crest and metastatic melanoma invasion and promotes collective migration in a manner consistent with inhibition of BMP signaling.

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Loss of neural crest-associated gene FOXD1 impairs melanoma invasion and migration via RAC1B downregulation

Project description:Recent studies suggest that malignant melanoma heterogeneity includes subpopulations of cells with features of multipotent neural crest (NC) cells. Zebrafish and mouse models have shown that reactivation of neural crest-specific pathways determines the invasiveness of melanoma cells. In this study, we show that the neural crest-associated transcription factor FOXD1 plays a key role in invasion and migration capacity of metastatic melanomas both in vivo and in vitro. Gene expression profiling analysis identified on one hand, an upregulation of FOXD1 in NC and melanoma cells, and on the other hand, a downregulation of several genes related to cell invasion in FOXD1 knockdown cells, including MMP9 and RAC1B. Furthermore, we demonstrate that knockdown of RAC1B a tumor-specific isoform of RAC1, significantly impaired cell migration and invasion in melanoma and could abrogate enhanced invasiveness induced by FOXD1 overexpression. We conclude that FOXD1 may influence invasion and migration via indirect regulation of MMP9 and RAC1B alternative splicing in melanoma cells.

2018-08-31 | GSE111766 | GEO

Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions.

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Loss of neural crest-associated gene FOXD1 impairs melanoma invasion and migration via RAC1B downregulation

Project description:Loss of neural crest-associated gene FOXD1 impairs melanoma invasion and migration via RAC1B downregulation

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Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-12-19 | GSE108230 | GEO

Cancer associated HIF-2a governs trunk neural crest stemness

Project description:We report that cancer associated protein HIF-2a is expressed in trunk neural crest neuroblastoma precursor cells in the developing embryo in three different species; human, mouse and avian. Dysregulation of HIF-2a leads to alterations in embryonic development, and neural crest cell migration, proliferation and self-renewal capacity. With RNAsequencing we report that alterations of HIF-2a expression affects the global transcriptome and that gene ontology enrich for the same processes observed in vivo.

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