Project description:Transcriptional response of the hyperthermophilic sulfate reducing archaeon Archaeoglobus fulgidus VC16 to lithoautotrohpic and heterotrophic growth conditions

2014-03-10 | E-MTAB-2294 | biostudies-arrayexpress

Assessment of the carbon monoxide metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus VC-16 by comparative transcriptome analyses

Project description:The metabolic versatile hyperthermophilic dissimilatory sulfate-reducing archaeon, Archaeoglobus fulgidus VC-16, both utilize carbon monoxide as energy source and is highly resistant to toxic effects of CO. This metabolic capacity was investigated by transcriptional response to growth with CO of cultures supplemented with sulfate (S-CO) or thiosulfate (T-CO), and without external electron acceptor (CO-without election acceptor ).

2015-04-30 | E-MTAB-3035 | biostudies-arrayexpress

Archaeoglobus fulgidus DSM 4304

Project description:Hyperthermophilic sulfate-reducing archaeon

| PRJNA104 | ENA

The crystal structure of the AF2331 protein from Archaeoglobus fulgidus DSM 4304 forms an unusual interdigitated dimer with a new type of alpha + beta fold.

Project description:The structure of AF2331, a 11-kDa orphan protein of unknown function from Archaeoglobus fulgidus, was solved by Se-Met MAD to 2.4 A resolution. The structure consists of an alpha + beta fold formed by an unusual homodimer, where the two core beta-sheets are interdigitated, containing strands alternating from both subunits. The decrease in solvent-accessible surface area upon dimerization is unusually large (3960 A(2)) for a protein of its size. The percentage of the total surface area buried in the interface (41.1%) is one of the largest observed in a nonredundant set of homodimers in the PDB and is above the mean for nearly all other types of homo-oligomers. AF2331 has no sequence homologs, and no structure similar to AF2331 could be found in the PDB using the CE, TM-align, DALI, or SSM packages. The protein has been identified in Pfam 23.0 as the archetype of a new superfamily and is topologically dissimilar to all other proteins with the "3-Layer (BBA) Sandwich" fold in CATH. Therefore, we propose that AF2331 forms a novel alpha + beta fold. AF2331 contains multiple negatively charged surface clusters and is located on the same operon as the basic protein AF2330. We hypothesize that AF2331 and AF2330 may form a charge-stabilized complex in vivo, though the role of the negatively charged surface clusters is not clear.

| S-EPMC2788295 | biostudies-literature

Archaeoglobus

Project description:Archaeoglobus sp. overview

| PRJNA687307 | ENA

Caridonax fulgidus

Project description:Caridonax fulgidus

| PRJNA920565 | ENA

Caridonax fulgidus

Project description:Caridonax fulgidus overview

| PRJNA924892 | ENA