Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression signature of TATA DNA binding mutant- spt15_A101T mutant (MVA pathway flux increasing mutant)

ABSTRACT: Gene expression signature of TATA DNA binding mutant- spt15_A101T mutant (MVA pathway flux increasing mutant)

PROVIDER: PRJNA427185 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression signature of TATA DNA binding mutant- spt15_A101T mutant (MVA pathway flux increasing mutant)

Project description:we applied the gene expression approach to identify the genes which are responsible for increasing the flux in MEV pathway in Saccharomyces cerevisiae by spt15_A101T

2018-12-30 | GSE108411 | GEO

Mot1 redistributes TBP from TATA-containing to TATA-less promoters

Project description:Mot1 redistributes TBP from TATA-containing to TATA-less promoters

| PRJNA189052 | ENA

Novel MVA Vector Expressing Anti-apoptotic gene, B13R, Delays Apoptosis and Enhances Humoral Responses

Project description:Modified vaccinia virus Ankara (MVA) has been explored as a vaccine vector for use against infectious diseases and cancer. MVA is an immunogenic, attenuated poxvirus capable of eliciting robust cellular and humoral responses in pre-clinical animal models and in patients. However, upon infection with MVA, cells undergo rapid apoptosis leading to faster clearance of recombinant antigens. The fragmentation of the anti-apoptotic gene B13R in MVA could contribute to this effect. Here, we replaced the fragmented B13R with a functional version and observed that MVA-B13R infected HeLa cells and muscle cell lines delayed caspase 3 activation compared to MVA indicating slower progression of apoptosis. For immunogenicity studies, mice were intramuscularly immunized with recombinant MVA or MVA-B13R expressing SIV Gag, Pol and HIV Env (SHIV). We observed higher Env-specific humoral responses from MVA-B13R SHIV compared to MVA SHIV mice. To determine differences in the innate immune response that may have contributed to the augmented humoral response, we performed RNA-Seq analysis on draining lymph node cells after immunization. Gene set enrichment analysis from day 1 after immunization showed that MVA-B13R SHIV immunizations were associated with a negative enrichment for type I and II interferon responses compared to MVA SHIV mice indicating MVA-B13R SHIV induces a delayed anti-viral interferon response that may lead to the enhanced humoral response observed. Taken together, these results demonstrate that restoring B13R functionality in MVA significantly delays MVA-induced apoptosis, augments Env-specific antibody responses, and is associated with reduced interferon-alpha and interferon-gamma responses induced after vaccination.

2018-11-27 | GSE119884 | GEO

EXP2. Antitumor effect MVA-Combo

Project description:RNA-seq analysis of ID8.OVA.Vegf/GFP omental metastases treated intraperitoneally with MVA expressing a tumor-associated antigen (TAA), CD40L and/or CD137L.

2022-07-19 | GSE196024 | GEO

Transcription profiling of Halobacterium salinarum wild type vs TATA-box mutant, deltaOE2375F, 6xHis-OE2375F

Project description:experiment is devided in 3 parts: I. comparison H.sal. R1 wt vs H.sal. R1 TATA-box mutant 3 microarrays belong to this objective II. comparison H.sal. R1 wt vs H.sal. R1 deltaOE2375F 2 microarrays belong to this objective III. comparison H.sal. R1 wt vs H.sal. R1 6xHis-OE2375F 1 microarray belongs to this objective

2005-11-01 | E-MEXP-327 | biostudies-arrayexpress

Mot1 redistributes TBP from TATA-containing to TATA-less promoters

Project description:The Swi2/Snf2-family ATPase Mot1 displaces TBP from DNA in vitro, but the global relationship between Mot1 and TBP in vivo has been unclear. We therefore mapped the distribution of Mot1 and TBP on native chromatin at base-pair resolution. Mot1 and TBP binding sites coincide throughout the genome, and depletion of TBP results in a global decrease in Mot1 binding. Using midpoint-versus-length mapping to assess the spatial relationship of Mot1 and TBP on chromatin, we find evidence that Mot1 approaches TBP from the upstream direction, consistent with its in vitro mode of action. Strikingly, inactivation of Mot1 leads to both increases and decreases in TBP-genome association. Sites of TBP gain tend to contain robust TATA boxes, while sites of TBP loss contain poly(dA:dT) tracts that may contribute to nucleosome exclusion. We propose that the action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites.

2013-10-21 | GSE44200 | GEO

EXP2. Antitumor effect MVA-Combo

Project description:EXP2. Antitumor effect MVA-Combo

| PRJNA803033 | ENA

Next Generation Sequencing Analysis of Wild Type and cGAS-/- BMDC infected with MVA or MVAΔE5R

Project description:Based on RNA-seq technology, we studies gene expression profiles in BMDC before and after MVA or MVAΔE5R infection. We identified genes which expression dependent on cGAS comparing WT and cGAS-/- cells after MVA infection. The difference genes bewteen MVA and MVAΔ5R were also identified.

2022-10-06 | GSE185431 | GEO

APOΕ4 lowers energy expenditure in females and impairs glucose oxidation by increasing flux through aerobic glycolysis

Project description:APOΕ4 lowers energy expenditure in females and impairs glucose oxidation by increasing flux through aerobic glycolysis

| PRJNA927342 | ENA

Intradermal but not intramuscular MVA immunizations protect against intravaginal tier2 SHIV challenges in macaques

Project description:Route of immunization can markedly influence the quality of immune response. Here, we show that intradermal (ID) but not intramuscular (IM) modified vaccinia Ankara (MVA) vaccinations provide protection from acquisition of intravaginal tier2 SHIV challenges in female macaques. Both routes of vaccination induced comparable levels of serum IgG with neutralizing and non-neutralizing activities. The protection in MVA-ID group correlated positively with serum neutralizing and antibody-dependent phagocytic activities, and envelope-specific vaginal IgA; while the limited protection in MVA-IM group correlated only with serum neutralizing activity. MVA-ID immunizations induced greater germinal center Tfh and B cell responses, reduced the ratio of Th1 to Tfh cells in blood and showed lower activation of intermediate monocytes and inflammasome compared to MVA-IM immunizations. This lower innate activation correlated negatively with induction of Tfh responses. These data demonstrate that the MVA-ID vaccinations protect against intravaginal SHIV challenges by modulating the innate and T helper responses.

2023-07-27 | GSE219118 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data