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RNA profiling of the liver and gut tissues in zebrafish (Danio rerio)

ABSTRACT: RNA profiling of the liver and gut tissues in zebrafish (Danio rerio)

PROVIDER: PRJNA427275 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6411470.fastq.gz	Fastqsanger.gz
	SRR6411471.fastq.gz	Fastqsanger.gz
	SRR6411472.fastq.gz	Fastqsanger.gz
	SRR6411465.fastq.gz	Fastqsanger.gz
	SRR6411466.fastq.gz	Fastqsanger.gz

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Project description:In triplicate for each condition, 12 WT and acbd6 F0 crispant Danio rerio (zebrafish) embryos were incubated with 20 μM YnMyr for 24 h, either between 48-72 hpf or 96-120 hpf. After labelling, zebrafish were washed twice with fresh egg water, deyolked, flash frozen in liquid nitrogen and stored at -80°C until further analysis.

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RNA profiling of the liver and gut tissues in zebrafish (Danio rerio) [mRNA]

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Zebrafish (Danio rerio) catecholaminergic neuron RNA-seq based transcript profiles

Project description:This project aimed at identifying developmental stage specific transcript profiles for catecholaminergic neurons in embryos and early larvae of zebrafish (Danio rerio). Catecholaminergic neurons were labeled using transgenic zebrafish strains to drive expression of GFP. At stages 24, 36, 72 and 96 hrs post fertilization, embryos were dissociated and GFP expressing cells sorted by FACS. Isolated RNAs were processed using either polyA selection and libray generation or NanoCAGE. This is the first effort to determine stage specific mRNA profiles of catecholaminergic neurons in zebrafish.

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