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Genome-wide profilings of EDM2-mediated effects on H3K9me2 and transcripts in Arabidopsis thaliana

ABSTRACT: Genome-wide profilings of EDM2-mediated effects on H3K9me2 and transcripts in Arabidopsis thaliana

PROVIDER: PRJNA427432 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6415930.fastq.gz	Fastqsanger.gz
	SRR6415940.fastq.gz	Fastqsanger.gz
	SRR6415931.fastq.gz	Fastqsanger.gz
	SRR6415941.fastq.gz	Fastqsanger.gz
	SRR6415932.fastq.gz	Fastqsanger.gz

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Genome-wide profilings of EDM2-mediated effects on H3K9me2 and transcripts in Arabidopsis thaliana

Project description:EDM2 is a PHD finger-containing histone binding protein in Arabidopsis thaliana (Arabidopsis) that affects levels of the transposon silencing mark H3K9me2. Here we report on genome-wide profiling by ChIP-seq and RNA-seq of EDM2-mediated effects on H3K9me2 and rRNA-depleted transcripts, respectively. EDM2 affects H3K9me2 and/or transcript levels at hundreds transposons and over 3000 gene loci including 60 NLR genes. In addition, EDM2 also binds to several NLR genes and transposons.

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Genome-wide profiling of EDM3 dependent H3K9me2 in Arabidopsis thaliana

Project description:We perform genome-wide profiling of H3K9me2 in the Arabidopsis thaliana edm3 mutant. By var-seq, we identified EDM3 as a nuclear-localized protein featuring a single RNA-recognition motif (RRM). Similar to PHD finger-containing histone binding protein EDM2, EDM3 promotes high levels of H3K9me2 at RPP7 and controls transcripts of this NLR gene by suppressing proximal polyadenylation and promoting the synthesis of full-length RPP7-coding mRNAs. Our results showed that EDM3 affects levels of this epigenetic mark at a set of genes and transposons, the vast majority of which also feature EDM2-dependent H3K9me2.

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Project description:The objective of the study is to profile histone H3 lysine nine di-methylation (H3K9me2) in Arabidopsis thaliana and to correlate it with DNA methylation.

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Genome wide profiling of H3K9me2 in Arabidopsis thaliana

Project description:The objective of the study is to profile histone H3 lysine nine di-methylation (H3K9me2) in Arabidopsis thaliana and to correlate it with DNA methylation. We constructed a high-resolution genome-wide map of H3K9me2 methylation by using native chromatin immunoprecipitation coupled with HD2 whole genome Nimblegen microarrays. Three replicas were performed for the native ChIP. As a control, one replica of ChIP isolated from crosslinked tissue was used.

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Arabidopsis AGDP1 links H3K9me2 to DNA methylation in heterochromatin

Project description:Heterochromatin is a tightly packed form of DNA, which is associated with histone 3 lysine 9 methylation (H3K9me). Here, we identify an H3K9me2 binding protein, Agenet domain (AGD)-containing protein 1 (AGDP1), in Arabidopsis thaliana. Our biochemical studies revealed that the AGDP1 can specifically recognize the H3K9me2 marks by the three pairs of tandem-AGDs. We determined the crystal structure of the AGD12 of Raphanus sativus AGDP1 in complex with an H3K9me2 peptide. In the complex, the histone peptide adopts a unique helical conformation. AGD12 employs a newly defined interacting interface to specifically recognize the H3K4me0 and H3K9me2 marks. In addition, we found that AGDP1 is required for transcriptional gene silencing, non-CG DNA methylation, and H3K9 dimethylation. Our ChIP-seq data showed that AGDP1 is enriched in TE regions and associated with the heterochromatin marks. Our findings suggest that, as a heterochromatin binding protein, AGDP1 links H3K9me2 to DNA methylation in heterochromatin regions.

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WGBS of Arabidopsis thaliana COM-edm2 mutant

Project description:Here we address that a mutation of EDM2 (Enhanced Downy Mildew 2), which is regarded as regulator of genome DNA methylation patterns, displays abnormal cotyledon number and shorted root length. Further, in early embryogenesis, edm2 shows embryonic developmental abnormalities, including the appearance of asymmetric embryos at the heart stage and unclear boundary between the spherical proembryo and suspensor cells. In addition, dysfunction of EDM2 alters the level of the auxin transporter PINs (PIN-FORMEDs), consistent with disordered distribution of auxin, which maybe the reason for developmental defect. Interestingly, analysis of high-throughput sequencing data show that the loss-of-function EDM2 exhibits the decreased expression of auxin polar transport-related gene PLT1 (PLETHORA 1), while methylation level and H3K9me2 enrichment in gene body are slightly higher than that in wide type. These results advance our understanding of EDM2 in establishment of auxin gradients through epigenetic modification and elucidate the roles of EDM2 in early embryogenesis.

2023-04-23 | GSE230021 | GEO

Expression data from 2-week-old Arabidopsis untreated seedlings grown under a short day condition

Project description:The Arabidopsis thaliana defense regulator EDM2 was previously shown to be specifically required for disease resistance to the pathogenic oomycete Hyaloperonospora parasitica aradidopsis mediated by the R protein RPP7. We found EDM2 to have a promoting effect on several distinct developmental processes, such as leaf pavement cell development, vegetative phase change or the floral transition. We further identified the atypical protein kinase WNK8 to physically interact with EDM2 in nuclei. Microarray data support regulatory interactions between WNK8 and EDM2 as well as their functional association with the autonomous floral promotion pathway.

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