Bacillales
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA428872 | ENA |
REPOSITORIES: ENA
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Project description:Due to the high capacity of their secretion machinery Gram-positive bacteria from the genus Bacillus are important expression hosts for the high-yield production of enzymes in industrial biotechnology. However, to date strains from only a few Bacillus species are used for enzyme production at the industrial scale. In this work, we introduce with Paenibacillus polymyxa DSM 292 a member of a different genus as a novel host for secretory protein production. The model gene cel8A from Clostridium thermocellum was chosen as an easily detectable reporter gene with industrial relevance to demonstrate efficient heterologous expression and secretion in P. polymyxa. The yield of the secreted cellulase Cel8A was increased by optimizing the expression medium and testing various promoter sequences on the expression plasmid pBACOV. To identify promising new promoter sequences from the genome of P. polymyxa itself, quantitative mass spectrometry was used to analyze the secretome. The most strongly secreted host proteins were identified and the promoters regulating the expression of the corresponding genes were selected. Eleven promoter sequences were cloned and tested, including well-characterized promoters from B. subtilis and B. megaterium. The best result was achieved with the promoter of the hypothetical protein PPOLYM_03468 from P. polymyxa, which in combination with the improved expression medium enabled the production of 5,475 U/l Cel8A which represents a 6.2-fold increase compared to the reference promoter PaprE. The set of promoters described in this work covers a broad range of promoter strengths useful for heterologous expression in the new host P. polymyxa.
2018-09-05 | PXD009882 | Pride
Project description:Strains of the Bacillales order isolated from various sources, including soil, marine sediment, rhizosphere, animal, plant, and fungi in four continents. Genome sequencing and assembly
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Project description:Helicobacter pylori, which is known as pathogens of various gastric diseases, have many types of genome sequence variants. That is part of the reason why pathogenesis and infection mechanisms of the H. pylori-driven gastric diseases have not been well clarified yet. Here we performed a large-scale proteome analysis to profile the heterogeneity of the proteome expression of 7 H. pylori strains by using LC/MS/MS-based proteomics approach combined with a customized database consisting of non-redundant tryptic peptide sequences derived from full genome sequences of 52 H. pylori strains. The non-redundant peptide database enabled us to identify more peptides in the database search of MS/MS data, compared with a simply merged protein database. Using the approach we performed proteome analysis of genome-unknown strains of H. pylori in as large-scale as genome-known ones. Clustering of the H. pylori strains using the proteome profiling slightly differed from the genome profiling and more clearly divided the strains into two groups based on the isolated area. Furthermore, we also identified phosphorylated proteins and sites of the H. pylori strains and obtained phosphorylation motif located in the N-terminus, which are commonly observed in bacteria.
2018-12-28 | PXD011364 | JPOST Repository
Project description:Aerobic endospore-forming bacteria genome sequencing and assembly
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Project description:Genome sequencing of bacteria isolated from Japanese honey
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Project description:Aerobic endospore-forming bacteria Genome sequencing and assembly
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Project description:Soil Bacterial Isolate from Order of Bacillales Genome Sequencing and Assembly
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Project description:Tellurium (Te) is a metalloid that is growing as a challenge since its increasing environmental presence, as a result of mining activity and improper disposal of high-tech devices, leads to an increasing selective pressure against living organisms. How microorganims adapt to the presence of increasing concentrations of metalloids, such as Te, could increase the know-how to solve Te contamination problems. Two mining isolates, Bacillus sp. and Paenibacillus sp, were able to grow in high concentrations of Te(IV) and were able to reduce Te(IV) to Te(0) so the effects of growth in the presence of Te(IV) was determined, at the level of total protein expression, for this strains. It was determined that growth of these strains in the presence of Te(IV) affected the general protein expression pattern, and hence the metabolism.
2022-02-15 | PXD017546 | Pride