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Transcriptional profiles of lung macrophage subsets in Mycobacterium Tuberculosis infection

ABSTRACT: Transcriptional profiles of lung macrophage subsets in Mycobacterium Tuberculosis infection

PROVIDER: PRJNA428925 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6493960.fastq.gz	Fastqsanger.gz
	SRR6493961.fastq.gz	Fastqsanger.gz
	SRR6493962.fastq.gz	Fastqsanger.gz
	SRR6493963.fastq.gz	Fastqsanger.gz
	SRR6493964.fastq.gz	Fastqsanger.gz

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Transcriptional profiles of lung macrophage subsets in Mycobacterium Tuberculosis infection

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Project description:Background: Tuberculosis (TB) remains a major public health problem, especially in developing countries, with 1.5 million deaths annually worldwide. Macrophages (Mφs) are central to TB pathogenesis. Mφs play a key role in the outcome of the infection. More importantly, Mφs are the primary cell target of MTB, which has developed different strategies to multiply inside the Mφ phagosome. Here we aim to compare the transcriptome of Mycobacterium tuberculosis (MTB)-infected Mφs with that of cells stimulated with heat-killed MTB. Results: Here, we used live and heat-inactivated MTB to examine the impact of infection on the human macrophage response. Gene expression profiling has revealed about 3,259 genes differentially expressed in macrophages incubated with heat-inactivated MTB and 4,179 genes differentially expressed with live MTB. We found that the response to live and heat-inactivated MTB was strongly correlated (r>0.78). Conclusions: Our results show that macrophages respond in a similar manner to live and heat-inactivated MTB. We also identified some genes differentially expressed only in one condition.

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