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The effect of productive HPV16 infection on the cervical epithelium transcriptome

ABSTRACT: The effect of productive HPV16 infection on the cervical epithelium transcriptome

PROVIDER: PRJNA429466 | ENA |

REPOSITORIES: ENA

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The effect of productive HPV16 infection on the cervical epithelium transcriptome

Project description:Microarray analysis of the effect of early stage, productive HPV16 infection on the transcriptome of human cervical keratinocytes. Our results provide for the first time, global gene expression changes in cervical epithelium that produces high levels of viral titers.

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Tissue-specific gene expression during productive HPV16 infection of cervical, foreskin and tonsil epithelium.

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circularRNA Sequencing of HPV16 positive cervical cancer tissues, HSIL tissues and normal cervical epithelial tissues

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Expression data of mRNA and circRNA from HPV16 positive cervical cancer tissues, HSIL tissues and normal cervical epithelial tissues

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Human Papillomavirus (HPV) genomic integration, expression and E6/E7 splicing patterns in cervical cancer associated with HPV16 or HPV18 infection

Project description:Human papillomavirus (HPV) genome integration into the host genome, blocking E2 expression and leading to overexpression of E6 and E7 viral oncogenes, is considered a major step in cervical cancer development. In high-risk HPVs, E6 and E7 oncogenes are expressed as a bicistronic pre-mRNA, with alternative splicing producing the ultimate mRNAs required for E6 and E7 translation. Given the number of alternative donor and acceptor splicing sites, ten E6/E7 different alternative transcripts might be formed for HPV16 and three for HPV18, although only six isoforms have been previously reported for HPV16. In the present work, we employ high-throughput sequencing of invasive cervical cancer transcriptome (RNA-Seq) to characterize the expression of the HPV genome in 24 invasive cervical cancers associated with HPV16 and HPV18 single infections. Based on high-resolution transcriptional maps, we herein report three viral gene expression patterns which might be associated with the presence of the viral genome in episomal and/or integrated stages. Alternative mRNAs splicing isoforms coding for E6 and E7 oncoproteins were characterized and quantified, and two novel isoforms were identified. Three major isoforms (E6*I, E6*II, and E6+E7) were detected for HPV16 and two for HPV18 (E6*I and E6+E7). Minor transcript isoforms, including the novel ones, were very rare in some tumor samples or were not detected. Our data suggested that minor transcript isoforms of E6/E7 do not play a relevant role in cervical cancer.

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GENE EXPRESSION PROFILE REGULATED BY THE HPV16-E7 ONCOPROTEIN AND ESTRADIOL IN CERVICAL TISSUE

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