Project description:Pao extract is an herbal preparation of the bark of an amazonian rain forest tree,Pao Pereira (Geissospermum vellosii),which could inhibit Benign prostatic hyperplasia (BPH).Characterizing the molecular alterations of BPH1 and WPMY-1 cells treated with PAO is important for understanding the molecular mechanisms of PAO inhibiting BPH. We used microarrays to detail the RNA expression.
Project description:Deep sequencing of the transcriptome of P. vivax parasite populations from vivax malaria patients with scarce parasitemia from the low transmission Brazilian Amazonian endemic region we the aim of better understanding the molecular mechanisms behind this cytoadherence and rosetting phenotypes by identifying proteins, especially parasitic ligands, which might be important for the P. vivax adhesion capacity within the human host. We used RNA-seq coupled with parasite enrichment from field samples and cytoadherence and rosetting assays to privilege the sequence of the whole transcriptome of parasite populations with distinct adhesive characteristics and, also assess the human host immune-related expression profile in the context of vivax malaria disease.
Project description:Climate change forecasts increase the susceptibility of forest due to longer drier seasons. The adaptive management protocols have highlighted the reduction of the forest densification to improve their vulnerability to extreme climate events (i.g. drought). One of this sensitive woody species to climate change is the Abies pinsapo, a relic conifer tree endemic from the southern Spain. Previous works have shown changes in their trends because of the climate change action, being carried out experimental thinning management in their lowest distribution limit, in Sierra de las Nieves Natural Park (Malaga). Our objective is to evaluate the water improvements of thinned trees in terms of light availability by means of a shading treatment in those thinned trees. To do that we have evaluated the synergic effect of ecophysiology, metabolomics and transcriptomics in control, thinning and thinning+shading plots in wet and dry seasons for two years. The results showed strong differences between summer and spring seasons at the three studied levels. The water deficit shows a greater influence than light exposure in the ecophysiology and metabolomics tree response. And the transcriptomics suggested an improvement of thinned trees when light exposure was reduced. Our results support the necessity of adaptive forest management in order to improve the conservation status of A. pinsapo forest. The combination of different levels of tree response is paramount to understand and predict the tree physiology under water and light stress conditions.
Project description:<p>A genome-wide analysis of genetic structure, gene flow, and natural selection was conducted in populations in the endemic Burkitt lymphoma (eBL) belt in Ghana and Northern Uganda, both subject to a high incidence of falciparum malaria and eBL. These populations have different ethnolinguistic ancestries and are located 2400 miles apart in sub-Saharan Africa. We characterized genetic composition of these populations in the context of 22 additional African populations and present evidence for gene flow events that occurred in the last 3000 years, possibly related to regional migrations in Western Africa and major migrations involving Nilotic, Cushitic, and Bantu groups. The Ugandan population was comprised of 758 children (mean age ~7 years) from 17 Western Nilo-Saharan tribes. The current dataset includes 561 healthy children and 197 children with eBL from northern Uganda.</p>
Project description:To better understand the response mechanisms against heat stress of tree peony, investigations of phenotypic changes, physiological responses, and quantitative proteomics were conducted
