Project description:Transcriptomic insight into differential gene expression patterns of the camel pathogenic nematode, Parabronema skrjabini, at specific developmental stages
Project description:Parabronema skrjabini is an widespread but neglected blood-feeding nematode. The P.skrjabini can ,as a pathogen ,causes the large ruminants clinical and pathological effects, with even severe infections being fatal, which is of a major veterinary importance in many developing countries. However, there is little information that controls the developmental changes of the parasite survived and developed in insect and ruminant hosts. Here we have collected P.sktjabini of four specific developmental stages and allows for complete understanding the key characteristics of development. We analyzed the transcriptomic changes accompanying the four specific developmental stages using RNA-seq and pinpointed the development-dependent function genes and biological processes.
Project description:To understand the regulatory regions of genomic DNA by nuclear pore, the genomic region associated by NUP153, one of nuclear pore protein, was determined by Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) .
