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Citrus suavissima breed:ougan | cultivar:seedless ougan

ABSTRACT: Citrus suavissima breed:ougan | cultivar:seedless ougan Raw sequence reads

PROVIDER: PRJNA430556 | ENA |

REPOSITORIES: ENA

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Comparative transcript profiling of gene expression between seedless Ponkan mandarin (Citrus reticulata Blanco) and its seedy wild type during floral organ development

Project description:Seedlessness is an important agronomic trait for citrus, and male sterility (MS) is one main cause of seedless citrus fruit. However, the molecular mechanism of citrus seedlessness remained not well explored. An integrative strategy combining SSH (suppression subtractive hybridization) library with cDNA microarray was employed to study the underlying mechanism of seedlessness of a Ponkan mandarin seedless mutant (Citrus reticulate Blanco). Screening with custom microarray, a total of 279 differentially expressed clones were identified, and 133 unigenes (43 contigs and 90 singletons) were obtained after sequencing. Gene Ontology (GO) distribution showed that the majority of differential genes involved in metabolic process, responded to stimulus and functioned as DNA/RNA binding, catalytic activity and oxidoreductase activity. Expression dynamics of some candidate clones revealed a gene encoding male sterility protein 2 was highly up-regulated, while several transcription factors (TFs) such as AP2/EREBP, MYB, WRKY, NAC and C2C2-GATA zinc-finger domain TFs were specifically down-regulated in the seedless mutant compared with the wild type. Our research highlighted some candidate pathways that participated in the citrus male gametophyte development and could be beneficial for seedless citrus breeding in the future.

2012-05-22 | GSE38094 | GEO

Comparative transcript profiling of gene expression between seedless Ponkan mandarin (Citrus reticulata Blanco) and its seedy wild type during floral organ development

2012-05-21 | E-GEOD-38094 | biostudies-arrayexpress

Citrus suavissima breed:ougan | cultivar:wuzi ougan

Project description:Citrus suavissima breed:ougan | cultivar:wuzi ougan Raw sequence reads

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Litchi chinensis cultivar:Seedless late

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Vitis vinifera cultivar:Crimson Seedless

Project description:Vitis vinifera cultivar:Crimson Seedless Raw sequence reads

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