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Single-cell gene expression data from Ventricular-Subventricular Zone (V-SVZ) cells

ABSTRACT: Single-cell gene expression data from Ventricular-Subventricular Zone (V-SVZ) cells

PROVIDER: PRJNA430900 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6497850_1.fastq.gz	Fastqsanger.gz
	SRR6497850_2.fastq.gz	Fastqsanger.gz
	SRR6497851_1.fastq.gz	Fastqsanger.gz
	SRR6497851_2.fastq.gz	Fastqsanger.gz
	SRR6497852_1.fastq.gz	Fastqsanger.gz

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RNA-sequencing gene expression data from FACS-purified Ventricular-Subventricular Zone (V-SVZ) and cortical cells from hGFAP::GFP mice.

Project description:Quiescent neural stem cells (NSCs) in the adult ventricular-subventricular zone (V-SVZ) undergo activation and divide to generate neurons and glia. Here we show that Platelet-derived Growth Factor Receptor beta (PDGFRβ) is expressed by quiescent and early activated adult V-SVZ NSCs, and maintains their quiescence. We further showed that selective deletion of PDGFRβ in adult V-SVZ NSCs leads to activation of quiescent NSCs. We performed RNA-seq on FACS-sorted V-SVZ quiescent, early activated and late activated NSCs, as well as cortical cells from adult 2-4 month old mice.

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Project description:We performed large-scale single cell profiling of dissociated V-SVZ and OB cells from the same animal carrying either the hGFAP::CreERT2; Rosa26LSL-TdTomato (GCERT2) reporter, or ratNes::FLPOER; Rosa26FSF-TdTomato (NesFLPO) reporter. Scope-Seq profiling was performed on dissociated V-SVZ cells from C57BL/6J animals.

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Jedi-1/MEGF12-mediated phagocytosis controls the pro-neurogenic properties of microglia in the ventricular-subventricular zone

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