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Transcriptome-wide view of endosomal mRNP transport [RNA-Seq]

ABSTRACT: Transcriptome-wide view of endosomal mRNP transport [RNA-Seq]

PROVIDER: PRJNA431397 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6506620.fastq.gz	Fastqsanger.gz
	SRR6506621.fastq.gz	Fastqsanger.gz
	SRR6506622.fastq.gz	Fastqsanger.gz
	SRR6506623.fastq.gz	Fastqsanger.gz
	SRR6506624.fastq.gz	Fastqsanger.gz

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Transcriptome-wide view of endosomal mRNP transport

Project description:Transcriptome-wide view of endosomal mRNP transport

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Transcriptome-wide view of endosomal mRNP transport [iCLIP ]

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Project description:This SuperSeries is composed of the SubSeries listed below.

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USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7

Project description:The endosomal system is a highly dynamic multifunctional organelle, whose complexity is regulated in part by reversible ubiquitylation. Despite the wide-ranging influence of ubiquitin in endosomal processes, relatively few enzymes utilizing ubiquitin have been described to control endosome integrity and function. Here we reveal the deubiquitylating enzyme (DUB) ubiquitin-specific protease 32 (USP32) as a powerful new player in this context. Loss of USP32 inhibits late endosome (LE) transport and recycling of LE cargos to the TGN, resulting in dispersion and swelling of the late compartment. Using SILAC-based ubiquitome profiling we identify the small GTPase Rab7—the logistical centerpiece of LE biology—as a substrate of USP32. Mechanistic studies reveal that LE transport effector RILP prefers ubiquitylation-deficient Rab7, while retromer-mediated LE recycling benefits from an intact cycle of Rab7 ubiquitylation. Collectively, our observations suggest that reversible ubiquitylation helps switch Rab7 between its various functions, thereby maintaining global spatiotemporal order in the endosomal system.

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Wnt directs the endosomal flux of LDL-derived cholesterol and lipid droplet homeostasis

Project description:The Wnt pathway, which controls crucial steps of the development and differentiation programs, has been proposed to influence lipid storage and homeostasis. In this paper, using an unbiased strategy based on high content genome-wide RNAi screens that monitored lipid distribution and amounts, we find that Wnt3a regulates cellular cholesterol. We show that Wnt3a stimulates the production of lipid droplets, and that this stimulation strictly depends on endocytosed, LDL-derived cholesterol and on functional early and late endosomes. We also show that Wnt signaling itself controls cholesterol endocytosis and flux along the endosomal pathway, which in turn modulates cellular lipid homeostasis. These results underscore the importance of endosome functions for LD formation and reveal a previously unknown cellular program controlling lipid storage and endosome transport under the control of Wnt signaling.

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The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs.

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