Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription start site analysis reveals widespread divergent transcription in D. melanogaster and core promoter-encoded enhancer activities

ABSTRACT: Transcription start site analysis reveals widespread divergent transcription in D. melanogaster and core promoter-encoded enhancer activities

PROVIDER: PRJNA431435 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6508010.fastq.gz	Fastqsanger.gz
	SRR6508011.fastq.gz	Fastqsanger.gz
	SRR6508012.fastq.gz	Fastqsanger.gz
	SRR6508013.fastq.gz	Fastqsanger.gz
	SRR6508014.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Transcription start site analysis reveals widespread divergent transcription in D. melanogaster and core promoter-encoded enhancer activities.

Project description:Mammalian gene promoters and enhancers share many properties. They are composed of a unified promoter architecture of divergent transcripton initiation and gene promoters may exhibit enhancer function. However, it is currently unclear how expression strength of a regulatory element relates to its enhancer strength and if the unifying architecture is conserved across Metazoa. Here we investigate the transcription initiation landscape and its associated RNA decay in Drosophila melanogaster. We find that the majority of active gene-distal enhancers and a considerable fraction of gene promoters are divergently transcribed. We observe quantitative relationships between enhancer potential, expression level and core promoter strength, providing an explanation for indirectly related histone modifications that are reflecting expression levels. Lowly abundant unstable RNAs initiated from weak core promoters are key characteristics of gene-distal developmental enhancers, while the housekeeping enhancer strengths of gene promoters reflect their expression strengths. The seemingly separable layer of regulation by gene promoters with housekeeping enhancer potential is also indicated by chromatin interaction data. Our results suggest a unified promoter architecture of many D. melanogaster regulatory elements, that is universal across Metazoa, whose regulatory functions seem to be related to their core promoter elements.

| S-EPMC6009668 | biostudies-literature

Transcription start site analysis reveals widespread divergent transcription in D. melanogaster and core promoter-encoded enhancer activities

Project description:Sequencing of 5' ends of RNA molecules from control and exosome-depleted S2 cells.

2018-03-22 | GSE109588 | GEO

Transcription start site identification in Bacillus subtilis

Project description:Transcription start site identification in Bacillus subtilis

| PRJEB35762 | ENA

Widespread microRNA degradation elements in target mRNAs can assist the encoded proteins

Project description:Widespread microRNA degradation elements in target mRNAs can assist the encoded proteins

| PRJNA741532 | ENA

Analysis of Drosophila melanogaster nascent transcription start sites

Project description:Analysis of Drosophila melanogaster nascent transcription start sites

| PRJNA838629 | ENA

AMPKα2 knockout mice and usage of alternative transcription start sites

Project description:Role of alternative transcription start sites in muscle growth and development remains largely undetermined. Our WTSS-seq (whole transcriptome start site sequencing) approach to capture 5'-end of RNAs clearly revealed alternative transcription start events responsible for increased muscle mass in AMPK α2 knockout mice.

2018-05-09 | GSE112325 | GEO

Differential Transcription Start Site Usage in Brain-related Samples

Project description:Differential Transcription Start Site Usage in Brain-related Samples

| PRJNA408204 | ENA

Differential Transcription Start Site Usage in Brain-related Samples

Project description:Differential Transcription Start Site Usage in Brain-related Samples

| PRJNA408205 | ENA

Transcription start site identification in wild type Escherichia coli

Project description:Transcription start site identification in wild type Escherichia coli

| PRJEB40769 | ENA

Acetyl-methyllysine marks chromatin at active transcription start sites

Project description:Acetyl-methyllysine marks chromatin at active transcription start sites