Project description:We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by N starvation (1 h) in rice roots. Transcriptomic analysis of rice roots revealed that the expression of 288 genes was differentially regulated (144 up, 144 down) by N starvation (1 h).
Project description:We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by glutamate in rice roots. Transcriptomic analysis of rice roots revealed that the expression of at least 122 genes involved in metabolism, transport, signal transduction, defense, and stress responses was rapidly induced by glutamate within 30 minutes.
Project description:We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by glutamine in rice roots. Transcriptomic analysis of rice roots revealed that the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses was rapidly induced by glutamine within 30 minutes.
Project description:We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by NH4NO3 in rice roots. Transcriptomic analysis of rice roots revealed that the expression of at least 158 genes involved in metabolism, transport, signal transduction, and stress responses was rapidly induced by NH4NO3 within 30 minutes.
Project description:We used GeneChip Rice Genome Array (Affymetrix, Santa Clara, CA, USA) to identify genes that were rapidly induced by glutamine in rice roots. Transcriptomic analysis of rice roots revealed that the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses was rapidly induced by glutamine within 30 minutes. Total RNA from –N and Gln-treated roots (2.5 mM Gln, 30 min) was extracted using a phenol extraction protocol. RNA samples from two biological repeats were sent to the Affymetrix Gene Expression Service Lab at Academia Sinica, Taipei, Taiwan (http://ipmb.sinica.edu.tw/affy/) for target preparation, and hybridization to the GeneChip Rice Genome Array.
Project description:Potassium (K+) is one of the most important nutrient ions in plant cells and play crucial roles in many plant physiological and developmental processes. K+ deficiency is the common abiotic stress in natural environment, which inhibits plant growth and reduces production of crops. We used microarrays to analyse the transcriptomic changes in rice roots after suffering K+ starvation at different times. The results of GO analysis showed that these transcriptionally changed genes mainly fell into the categories of metabolic process, membrane, cation binding, kinase activity, transport and so on.
Project description:Rice roots grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray
Project description:Root architectural plasticity enables rice to adapt to fluctuating soil water conditions. PP2CABA is an abscisic acid-inducible clade F2 protein phosphatase that regulates rice root development, lignin and suberin deposition, and adaptation to drought and flooding stress. We used Affymetrix Rice GeneChip microarrays to compare transcriptomic profiles between wild-type rice roots and roots of XVE:PP2CABA-dHA transgenic rice seedlings following β-estradiol induction. This analysis was performed to identify genes and pathways regulated by PP2CABA in rice roots, particularly those associated with cell wall modification, phenylpropanoid biosynthesis, fatty acid elongation, and lipid transfer.