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Drosophila pandora

ABSTRACT: Drosophila pandora Raw sequence reads

PROVIDER: PRJNA432097 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR6655860_1.fastq.gz	Fastqsanger.gz
	SRR6655860_2.fastq.gz	Fastqsanger.gz
	SRR6655861_1.fastq.gz	Fastqsanger.gz
	SRR6655861_2.fastq.gz	Fastqsanger.gz
	SRR6655862_1.fastq.gz	Fastqsanger.gz

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Project description:Small non-coding RNAs (sncRNAs) are key molecules regulating gene expression. High-throughput RNA-seq greatly advanced sncRNA discovery; however, traditional cDNA library construction protocols generate biased sequencing results, in part due to RNA modifications that interfere with adapter ligation and reverse transcription processes, preventing the detection of sncRNAs bearing these modifications. Here, we present PANDORA-seq (Panoramic RNA Display by Overcoming RNA modification Aborted Sequencing), employing a combination of enzymatic treatments to remove key RNA modifications that block adapter ligation and reverse transcription during cDNA library construction. PANDORA-Seq enables the discovery of thousands of modified sncRNAs previously undetected, mostly tRNA-derived small RNAs (tsRNAs) and rRNA-derived small RNAs (rsRNAs), which are tissue/cell-specifically detected across mouse brain, liver, spleen, sperm, mouse and human embryonic stem cells, and HeLa cells. Moreover, PANDORA-Seq reveals dynamic changes of tsRNAs and rsRNAs during reprogramming of induced pluripotent stem cells (iPSCs), pointing to future investigations on their potential regulatory functions.

2021-02-16 | GSE144666 | GEO