Project description:We reported transcriptome profiles of rifamycin SV-produced strain Amycolatopsis mediterranei U32 in the Bennet medium with or without 80 mM nitrate in different cultivation stage. By comparative analysis of genome-wide gene expression in these conditions, we found that the mechanism of high production of rifamycin SV induced by nitrate could be elucidated.
Project description:Here we report the effect of two comparative transcriptomics experiments effect on EDDS producing biosynthetic gene cluster. The goals of this study are to compare RNA sequencing derived transcriptomes of Amycolatopsis japonica at 24 hours in zinc treated and control culture conditions in combination with control and zur regulator deleted strains, in order to target other genes for the overexpression of EDDS cluster.
Project description:We reported transcriptome profiles of rifamycin SV-produced strain Amycolatopsis mediterranei U32 in the Bennet medium with or without 80 mM nitrate in different cultivation stage. By comparative analysis of genome-wide gene expression in these conditions, we found that the mechanism of high production of rifamycin SV induced by nitrate could be elucidated. Examination of 2 time-course transcription in 2 different nitrogen source conditions
