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High-throughput sequencing identify miRNAs involved in exosomes of MDA-MB-231 cocultivated with or without mature 3T3-L1

ABSTRACT: High-throughput sequencing identify miRNAs involved in exosomes of MDA-MB-231 cocultivated with or without mature 3T3-L1

PROVIDER: PRJNA432230 | ENA |

REPOSITORIES: ENA

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	SRR6653346.fastq.gz	Fastqsanger.gz

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High-throughput sequencing identify miRNAs involved in exosomes of MDA-MB-231 cocultivated with or without mature 3T3-L1

Project description:To identify miRNAs involved in exosomes from MDA-MB-231 cultivated with mature 3T3-L1, small RNA libraries from exosomes stored at 20 °C for 0 and 24 h were constructed. A total of 432 small RNA sequences were generated, and 88 known and 1224 new candidate miRNAs were obtained. Among them, 85 miRNAs were up-regulated and 300 were down-regulated in exosomes from MDA-MB-231 cultivated with mature 3T3-L1.

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Gene expression profiles of human breast cancer cell lines MCF-7, T47D and MDA-MB-231 co-cultured with 3T3-L1 adipocytes.

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Project description:Dicer, RNase III endonuclease, is an essential enzyme in miRNA biogenesis that regulates target gene expression, and it has been reported that aberrant expressions of Dicer associate with the clinical outcomes of patients in various cancers. To explore the miRNA differencial expression regulated by Dicer in MDA-MB-231/E1A cells, the microarray profiling analysis was employed to conduct differentially expressed miRNAs in stable MDA-MB-231/vector, MDA-MB-231/E1A, and MDA-MB-231/E1A/shDicer cells.

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