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The probiotic Escherichia coli strain Nissle 1917 combats the lambdoid bacteriophages stx and λ

ABSTRACT: The probiotic Escherichia coli strain Nissle 1917 combats the lambdoid bacteriophages stx and λ

PROVIDER: PRJNA432361 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6658272.fastq.gz	Fastqsanger.gz
	SRR6658273.fastq.gz	Fastqsanger.gz
	SRR6658274.fastq.gz	Fastqsanger.gz
	SRR6658275.fastq.gz	Fastqsanger.gz
	SRR6658276.fastq.gz	Fastqsanger.gz

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The probiotic Escherichia coli strain Nissle 1917 combats the lambdoid bacteriophages stx and λ

Project description:The Escherichia coli strain Nissle 1917 (EcN) is used as a probiotic for the treatment of certain gastrointestinal diseases in several European and non-European countries. In vitro studies showed EcN to efficiently inhibit the production of Shiga toxin (Stx) by Stx producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC). The occurrence of the latest EHEC serotype (O104:H4) responsible for the great outbreak in 2011 in Germany was due to the infection of an enteroaggregative E. coli by a Stx 2-encoding lambdoid phage turning this E. coli into a lysogenic and subsequently into a Stx producing strain. Since EHEC infected persons are not recommended to be treated with antibiotics, EcN might be an alternative medication. However, because a harmless E. coli strain might be converted into a Stx-producer after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN towards not only stx-phages but also against the lambda phage. This resistance was not based on the lack of or by mutated phage receptors. Rather the expression of certain genes (superinfection exclusion B (sieB) and a phage repressor (pr) gene) of a defective prophage of EcN was involved in the complete resistance of EcN to infection by the stx- and lambda phage. Obviously, EcN cannot be turned into a Stx producer. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.

2018-06-14 | GSE109932 | GEO

Caco-2 cells cocultured with E.coli Nissle 1917

Project description:Confluent Caco-2 cells cultured over 6 hours non-treated with E.coli Nissle 1917 (GSM40938 and GSM40941). Confluent Caco-2 cells cocultured over 6 hours with E.coli Nissle 1917 (GSM40881 and GSM40937). Keywords: parallel sample

2005-03-02 | GSE2232 | GEO

Transcription profiling of human Caco-2 cells cocultured with E.coli Nissle 1917

Project description:Confluent Caco-2 cells cultured over 6 hours non-treated with E.coli Nissle 1917; (GSM40938 and GSM40941). Confluent Caco-2 cells cocultured over 6 hours with E.coli Nissle 1917 (GSM40881 and GSM40937).

2008-06-12 | E-GEOD-2232 | biostudies-arrayexpress

The Probiotic Escherichia coli Strain Nissle 1917 Combats Lambdoid Bacteriophages stx and λ.

Project description:Shiga toxin (Stx) producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC) are the major cause of foodborne illness in humans. In vitro studies showed the probiotic Escherichia coli strain Nissle 1917 (EcN) to efficiently inhibit the production of Stx. Life threatening EHEC strains as for example the serotype O104:H4, responsible for the great outbreak in 2011 in Germany, evolutionary developed from certain E. coli strains which got infected by stx2-encoding lambdoid phages turning the E. coli into lysogenic and subsequently Stx producing strains. Since antibiotics induce stx genes and Stx production, EHEC infected persons are not recommended to be treated with antibiotics. Therefore, EcN might be an alternative medication. However, because even commensal E. coli strains might be converted into Stx-producers after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN toward not only stx-phages but also against lambda-phages. This resistance was not based on the lack of or by mutated phage receptors. Rather it involved the expression of a phage repressor (pr) gene of a defective prophage in EcN which was able to partially protect E. coli K-12 strain MG1655 against stx and lambda phage infection. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.

| S-EPMC5987069 | biostudies-literature

E. coli Nissle 1917 transcriptional response after treatment with Calprotectin

Project description:To determine whether calprotectin can elicit any transcriptional response in the probiotic E. coli Nissle 1917(EcN), EcN was treated with 200 ug/g of calprotectin in log phase.

2023-08-16 | GSE211418 | GEO

Escherichia coli Nissle 1917

Project description:Escherichia coli Nissle 1917 proteome

| PRJNA71129 | ENA

RNA sequencing of Escherichia coli Nissle 1917 after HOCl stress

Project description:RNA sequencing of Escherichia coli Nissle 1917 before and after HOCl treatment was perfomed to identify pathways that may be important in responding to oxidative stress caused by reachive chlorine species (RCS).

2020-01-23 | GSE144068 | GEO

Comparative genomic hybridization by array of E. coli strains CFT073, Nissle 1917 and ABU 83872

Project description:CGH analysis of CFT073, Nissle 1917 and ABU 83972

2010-06-09 | E-MTAB-212 | biostudies-arrayexpress

Escherichia coli Nissle 1917

Project description:Escherichia coli Nissle 1917 Genome sequencing

| PRJNA447975 | ENA

Escherichia coli Nissle 1917

Project description:Escherichia coli Nissle 1917 Genome sequencing

| PRJNA1176130 | ENA