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A stable mode of bookmarking by TBP recruits RNAPolymerase II to mitotic chromosomes

ABSTRACT: A stable mode of bookmarking by TBP recruits RNAPolymerase II to mitotic chromosomes

PROVIDER: PRJNA432429 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6660120_1.fastq.gz	Fastqsanger.gz
	SRR6660120_2.fastq.gz	Fastqsanger.gz
	SRR6660130.fastq.gz	Fastqsanger.gz
	SRR6660121_1.fastq.gz	Fastqsanger.gz
	SRR6660121_2.fastq.gz	Fastqsanger.gz

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A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes.

Project description:Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in mouse embryonic stem cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes, in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery promotes efficient transcription maintenance globally.

| S-EPMC6037474 | biostudies-literature

A stable mode of bookmarking by TBP recruits RNAPolymerase II to mitotic chromosomes

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-07-04 | GSE109964 | GEO

TBP in mitotic bookmarking and reactivation of transcription [ChIP-Seq]

Project description:TBP in mitotic bookmarking and reactivation of transcription [ChIP-Seq]

| PRJNA432433 | ENA

Interaction between TBP and condensing drives the organization and faithful segregation of mitotic chromosomes

Project description:Genome/chromosome organization is highly ordered and controls nuclear events. Here, we show that the TATA box-binding protein (TBP) interacts with the Cnd2 kleisin subunit of condensin to mediate interphase and mitotic chromosome organization in fission yeast. TBP recruits condensin onto RNA polymerase III-transcribed (Pol III) genes and highly transcribed Pol II genes; condensin in turn associates these genes with centromeres. Inhibition of the Cnd2-TBP interaction disrupts condensin localization across the genome and the proper assembly of mitotic chromosomes, leading to severe defects in chromosome segregation and eventually causing cellular lethality. We propose that the Cnd2-TBP interaction coordinates transcription with chromosomal architecture by linking dispersed gene loci with centromeres. This chromosome arrangement can contribute to the efficient transmission of physical force at the kinetochore to chromosomal arms, thereby supporting the fidelity of chromosome segregation. Genome-wide distributions of condensin and Pol III factors in fission yeast.

2015-08-12 | E-GEOD-60273 | biostudies-arrayexpress

Interaction between TBP and condensing drives the organization and faithful segregation of mitotic chromosomes

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TBP in mitotic bookmarking and reactivation of transcription [ChIP-Seq]

Project description:To test of TBP binds in the same genomic regions in mitosis compared to interphase cells, we performed Chromatin ImmunoPrecipitation coupled with sequencing (ChIP-seq).

2018-07-04 | GSE109962 | GEO

TBP bookmarks and preserves neural stem cell fate memory by orchestrating local chromatin architecture [CUT&Tag]

Project description:Mitotic bookmarking has been posited as an important strategy for cells to faithfully propagate their fate memory though cell generations. However, the physiological significance and regulatory mechanisms of mitotic bookmarking in neural development remain unexplored. Here, we identified TBP (TATA binding protein) as a crucial mitotic bookmarker for preserving fate memory of Drosophila neural stem cells (NSCs). Phosphorylation by the super elongation complex (SEC) is important for TBP to retain as discrete foci at mitotic chromosomes of NSCs to effectively transmit their fate memory. TBP depletion leads to drastic NSC loss, whereas TBP overexpression enhances the ability of SEC to induce neural progenitor dedifferentiation and tumorigenesis. Importantly, TBP achieves its mitotic retention through recruiting the chromatin remodeler EP400, which in turn increases local chromatin accessibility via depositing H2A.Z. Thus, local chromatin remodeling ensures mitotic bookmarking, which may represent a general principle underlying the preservation of cell fate memory.

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TBP bookmarks and preserves neural stem cell fate memory by orchestrating local chromatin architecture [ATAC-seq]

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TBP bookmarks and preserves neural stem cell fate memory by orchestrating local chromatin architecture (ATAC-Seq)

2024-12-10 | GSE267891 | GEO

TBP bookmarks and preserves neural stem cell fate memory by orchestrating local chromatin architecture (RNA-Seq)

2024-12-10 | GSE267893 | GEO