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Role of TBP in reactivation of transcription following mitosis [RNA-Seq]

ABSTRACT: Role of TBP in reactivation of transcription following mitosis [RNA-Seq]

PROVIDER: PRJNA432434 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6660130.fastq.gz	Fastqsanger.gz
	SRR6660131.fastq.gz	Fastqsanger.gz
	SRR6660122.fastq.gz	Fastqsanger.gz
	SRR6660132.fastq.gz	Fastqsanger.gz
	SRR6660123.fastq.gz	Fastqsanger.gz

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Role of TBP in reactivation of transcription following mitosis [RNA-Seq]

Project description:We performed nascent chromatin-associated RNA analysis as a time course following mitosis to determine how transcription is reactivated globally as cells enter G1.

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TBP in mitotic bookmarking and reactivation of transcription [ChIP-Seq]

Project description:To test of TBP binds in the same genomic regions in mitosis compared to interphase cells, we performed Chromatin ImmunoPrecipitation coupled with sequencing (ChIP-seq).

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Project description:During mitosis, TATA-binding protein(TBP), which remains bound to DNA during mitosis, recruits PP2A and also interacts with a subunit of the condensin complex to allow efficient dephosphorylation and inactivation of condensin near these promoters to inhibit their compaction. These result suggest that TBP function is not only important for expression of genes transcribed bDuring mitosis, TATA-binding protein(TBP), which remains bound to DNA during mitosis, recruits PP2A and also interacts with a subunit of the condensin complex to allow efficient dephosphorylation and inactivation of condensin near these promoters to inhibit their compaction. These result suggest that TBP function is not only important for expression of genes transcribed by all three RNA polymerase during interphase, but also for transmitting the memory of gene activity through mitosis to daughter cellsy all three RNA polymerase during interphase, but also for transmitting the memory of gene activity through mitosis to daughter cells We use ChIP-chip approach to identify TBP-bindind sites in mitotic Jurkat cells at genome-wide level. Input DNA fragments and DNA fragments immunoprecipitated by TBP antibodies by ChIP assay on mitotic Jurkat cells were used to probe the Affymetrix Genechip human tiling 2.0c to identift TBP-binding sites in mitotic Jurkat cells. Input and ChIP sample were replicated three times as following: Input rep1, TBP ChIP1, Input rep2, TBP ChIP2, Input rep3, TBP ChIP3.

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Distinct modes of heat shock transcription factor interactions with mitotic chromosomes

Project description:TBP is required for efficient reactivation of Pol I following mitosis but is generally not required

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TBP ChIP-chip on Jurkat cells

2009-05-28 | GSE12256 | GEO

TBP in mitotic bookmarking and reactivation of transcription [ChIP-Seq]

Project description:TBP in mitotic bookmarking and reactivation of transcription [ChIP-Seq]

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Role of TBP in chromatin accessibility (ATAC-seq)

Project description:Role of TBP in chromatin accessibility (ATAC-seq)

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Role of TBP in chromatin accessibility (ATAC-seq)

Project description:To test the role of TBP in promoting chromatin accessibility through the cell cycle, we performed ATAC-seq analysis.

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A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition

Project description:A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition

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Multiple modes of regulation control dynamic transcription patterns during the mitosis-G1 transition

Project description:Following cell division, genomes must reactivate gene expression patterns that reflect the identity of the cell. Here, we use PRO-seq to examine the mechanisms that reestablish transcription patterns after mitosis. We uncover regulation of the transcription cycle at multiple steps including initiation, promoter-proximal pause positioning and escape, poly-A site cleavage and termination during the mitotic-G1 transition. During mitosis, RNA polymerase activity is retained at initiation sites, albeit shifted in position relative to non-mitotic cells. This activity is strongly linked to maintenance of local chromatin architecture during mitosis and is more predictive of rapid gene reactivation than histone modifications previously associated with bookmarking. These molecular bookmarks, combined with sequence-specific transcription factors, direct expression of select cell growth and cell specific genes during mitosis followed by reactivation of functional gene groups with distinct kinetics after mitosis. This study details how dynamic regulation of transcription at multiple steps contributes to gene expression during the cell cycle.

2022-04-15 | GSE179576 | GEO

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