Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human cytotrophoblast organoids

ABSTRACT: Human cytotrophoblast organoids

PROVIDER: PRJNA432503 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6661120.fastq.gz	Fastqsanger.gz
	SRR6661111.fastq.gz	Fastqsanger.gz
	SRR6661121.fastq.gz	Fastqsanger.gz
	SRR6661112.fastq.gz	Fastqsanger.gz
	SRR6661122.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Human cytotrophoblast organoids

Project description:Human cytotrophoblast organoid cultures were established from the villous trophoblast of first trimester placentas. We analyzed the global expression profile of the cytotrophoblast organoids (CTB-ORG) and compared to the profile of the tissue of origin i.e. villous cytotrophoblast (vCTB) as well as to differentiated syncytiotrophoblast (STB) and placental fibroblasts (FIB).

2018-08-02 | GSE109976 | GEO

Primary term human cytotrophoblast, untreated [ChIP-Seq]

Project description:Primary term human cytotrophoblast, untreated [ChIP-Seq]

| PRJNA510668 | ENA

Human endometrial organoids

Project description:Human endometrial organoids

| PRJNA528264 | ENA

Primary term human cytotrophoblast, untreated [ChIP-Seq]

Project description:Chromatin alterations are important mediators of gene expression changes. We have recently shown that activated non-canonical NF-κB signaling (RelB/p52) recruits histone acetyltransferase CBP and deacetylase HDAC1 to selectively acetylate H3K9 (H3K9ac) to induce expression of corticotropin-releasing hormone (CRH) and prostaglandin-endoperoxide synthase-2 (PTGS2) in the human placenta. Both of these genes play a role in initiating. We performed chromatin immunoprecipitation followed by gene sequencing (ChIP-seq) in primary term human cytotrophoblast (CTB) with use of antibodies to RelB, CBP, HDAC1 and H3K9ac. We further associated these chromatin alterations with gene expression changes from mid-trimester to term in CTB by RNA sequencing (RNA-seq). We detected a genome-wide differential gene enrichment between mid-trimester and term human placenta. Pathway analysis identified that cytokine-cytokine receptor interaction, NF-κB, and TNF are the leading pathways enriched in term placenta and associated with these chromatin alterations. Our analysis has provided the first-time characterization of the key players of human placental origin with molecular changes resulting from chromatin modifications, which could drive human labor.

2018-12-19 | GSE124080 | GEO

Derivation of a CDX2+/p63+ human cytotrophoblast stem cell from pluripotent stem cells in defined conditions

Project description:We show that WA09 human embryonic stem cells (hESC) cultured in minimal media and treated with BMP4 for 4 days differentiate towards a cytotrophoblast (CTB)-like state.

2019-11-28 | GSE65654 | GEO

Stem cell-derived trophoblast organoids model human placental development and susceptibility to emerging pathogens

Project description:Trophoblast organoids derived from placental villi provide a 3D model system of human placental development, but access to first-trimester tissues is limited. Here we report that trophoblast stem cells isolated from naïve human pluripotent stem cells (hPSCs) can efficiently self-organize into 3D stem cell-derived trophoblast organoids (SC-TOs) with a villous architecture similar to primary trophoblast organoids. Single cell transcriptome analysis reveals the presence of distinct cytotrophoblast and syncytiotrophoblast clusters and a small cluster of extravillous trophoblasts, which closely correspond to trophoblast identities in the post-implantation embryo. These organoid cultures display clonal X chromosome inactivation patterns previously described in the human placenta. We further demonstrate that SC-TOs exhibit selective vulnerability to emerging pathogens (SARS-CoV-2 and Zika virus), which correlates with expression levels of their respective entry factors. The generation of trophoblast organoids from naïve hPSCs provides an accessible 3D model system of the developing placenta and its susceptibility to emerging pathogens.

2022-04-29 | GSE172241 | GEO

Preeclampsia: the in vivo milieu leads to cytotrophoblast dysregulation

Project description:During human pregnancy, placental cytotrophoblasts invade the uterus and its blood vessels, anchoring the progeny and rerouting maternal blood to the embryo/fetus. In preeclampsia, cytotrophoblast invasion is restricted and blood flow to the placenta is reduced. The causes of restricted cytotrophoblast invasion are unknown. Here, preeclampsia and control cytotrophoblasts were cultured for 48 h to allow differentiation/invasion. In various severe forms of preeclampsia Â± intrauterine growth restriction, global transcriptional profiling revealed common aberrations in cytotrophoblast gene expression that resolved with culture. Villous cytotrophoblasts were isolated from preeclampsia placentas (PRE, n=5) and placentas of preterm labor patients without signs of infection (PTL, n=5), which served as gestation-matched controls. To better understand the CTB phenotype in the context of PE variants, we included patients with the most clinically significant forms of this condition that necessitated preterm delivery: women with severe PE Â± intrauterine growth restrictions, PE with superimposed hypertension and HELLP syndrome (hemolysis, elevated liver enzymes; low platelet count). RNA was purified immediately after the cells were isolated (0 h) and after 12, 24 and 48 h in culture. The relative gene expression across the whole genome was profiled using the Affymetrix HG-U133Plus 2.0 GeneChip platform. Array quality was assesed using RMAExpress. One sample of preterm labor collected at 48h was omitted (39 arrays total). We used both LIMMA and maSigPro (R/Bioconductor) to determine differentially expressed genes.

2012-08-17 | E-GEOD-40182 | biostudies-arrayexpress

Primary mid-trimester and term human cytotrophoblast, untreated [RNA-Seq]

Project description:Primary mid-trimester and term human cytotrophoblast, untreated [RNA-Seq]

| PRJNA511458 | ENA

Human villous cytotrophoblast: Rosiglitazone treated (RT-VCT) vs. Control (VCT)

Project description:Human villous cytotrophoblast: Rosiglitazone treated (RT-VCT) vs. Control (VCT)

| PRJNA565426 | ENA

Single cell assessment of trophoblast stem cell and primary cytotrophoblast derived organoids as placenta models

Project description:Single cell assessment of trophoblast stem cell and primary cytotrophoblast derived organoids as placenta models

| PRJNA892642 | ENA