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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [RNA-seq]

ABSTRACT: Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [RNA-seq]

PROVIDER: PRJNA432600 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6664520.fastq.gz	Fastqsanger.gz
	SRR6664521.fastq.gz	Fastqsanger.gz
	SRR6664522.fastq.gz	Fastqsanger.gz
	SRR6664523.fastq.gz	Fastqsanger.gz
	SRR6664524.fastq.gz	Fastqsanger.gz

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

Project description:Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [ChIP-seq]

Project description:Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [ChIP-seq]

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Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [RNA-seq]

Project description:Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3–4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.

2018-02-11 | GSE110036 | GEO

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [ChIP-seq]

2018-02-11 | GSE110039 | GEO

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts.

Project description:Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.

| S-EPMC5834688 | biostudies-literature

Derivation of formative-like pluripotent stem cells from mammalian embryos [ChIP-Seq]

Project description:Two phases of pluripotency, naïve and primed, have been captured in vitro and studied in details1. A third formative phase was recently proposed to exist between naïve and primed phases2. Formative pluripotency entails permissiveness for direct primordial germ cell (PGC) induction and competence for blastocyst chimeras, and is characterized by transcriptional and epigenetic features intermediate of naïve and primed pluripotency. To date, however, stable pluripotent stem cells (PSCs) harboring formative features haven’t been derived from early mammalian embryos. Here we develop a method which enabled the derivation and culture of stable formative-like embryonic stem cells (ESCs) from mouse blastocysts. Formative-like mouse ESCs share molecular features characteristic of early post-implantation epiblasts and are competent for PGC-like cell induction and blastocyst chimera formation. The same culture also supported the derivation of ESCs and transgene-free induced pluripotent stem cells (iPSCs) from horse blastocysts and fibroblasts, respectively. Horse ESCs/iPSCs transcriptionally resembled mouse formative cells, and could also be directly induced into PGC-like cells. Formative-like horse iPSCs could efficiently chimerize horse, mouse, goat, sheep and pig embryos. Stable formative-like PSCs will be invaluable for studying mammalian pluripotency, and our method may be broadly applicable for the derivation of PGC and chimera competent PSCs from other mammalian species.

2020-11-27 | GSE135990 | GEO

Derivation of formative-like pluripotent stem cells from mammalian embryos [RNA-Seq]

2020-11-27 | GSE135989 | GEO

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-02-11 | GSE110040 | GEO

Derivation of Porcine Extraembryonic Endoderm-like Cells from from Blastocysts

Project description:Derivation of Porcine Extraembryonic Endoderm-like Cells from from Blastocysts

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Modulation of Wnt and Activin/Nodal supports efficient derivation, cloning and suspension expansion of human pluripotent stem cells

Project description:Various culture systems have been used to derive and maintain human pluripotent stem cells (hPSCs), but they are inefficient in sustaining cloning and suspension expansion of hPSCs. Through systematically modulating Wnt and Activin/Nodal signaling, we developed a defined medium (termed AIC), which enables efficient cloning and long-term expansion of hPSCs (AIC-hPSCs) through single-cell passage on feeders, matrix or in suspension (25-fold expansion in 4 days) and maintains genomic stability of hPSCs over extensive expansion. Moreover, the AIC medium supports efficient derivation of hPSCs from blastocysts or somatic cells under feeder-free conditions. Compared to conventional-culture hPSCs, AIC-hPSCs have similar gene expression profiles but down-regulated differentiation genes and display higher metabolic activity. The AIC medium shows a good compatibility for suspension expansion of different hPSC lines. Our study provides a robust culture system for derivation, cloning and suspension expansion of high-quality hPSCs that benefits GMP production and processing of therapeutic hPSC products

2020-04-23 | GSE145163 | GEO

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