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Differential expression of mRNAs in CAL-51 cells transfected with microRNA negative control and miR-4306 mimic

ABSTRACT: Differential expression of mRNAs in CAL-51 cells transfected with microRNA negative control and miR-4306 mimic

PROVIDER: PRJNA433167 | ENA |

REPOSITORIES: ENA

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Differential expression of mRNAs in CAL-51 cells transfected with microRNA negative control and miR-4306 mimic

Project description:The possible downstream pathways of miR-4306 were analyzed, using Agilent mRNA microarray. CAL-51 cells treated with miR-4306 mimic were used to compare with the control group.

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CAL-51 breast cancer side population cells

Project description:Human solid tumors contain rare cancer side population (SP) cells, which expel the fluorescencent dye H33342 and display cancer stem cell characteristics. Transcriptional profiling of cancer SP cells isolated by H33342 fluorescence analysis is a newly emerging approach to discover cancer stem cell markers and aberrant differentiation pathways. Using Affymetrix expression microarrays this study investigated differential gene expression between SP and non-SP (NSP) cells isolated from the CAL-51 human mammary carcinoma cell line. Keywords: cell type comparison To characterize differential gene expression between CAL-51 breast cancer SP and NSP cells, three consecutive cell culture passages of CAL-51 were independently subjected to H33342 labeling and dual wavelength fluorescence analysis and were then flow cytometrically sorted into SP and NSP cell fractions. Subsequently, each of the six cell preparations was subjected to global transcriptional profiling using Affymetrix HG U133 Plus 2.0 expression microarrays.

2009-12-31 | E-GEOD-18773 | biostudies-arrayexpress

CAL-51 breast cancer side population cells

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RNAseq Study in CC-671 Treated Cal-51 Cells

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mRNA profiling of MCF7 cells transfected with a miR-139-5p mimic

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Differential expression of mRNAs in MDA-MB-231 cells transfected with microRNA negative control and miR-4306 mimic

Project description:Differential expression of mRNAs in MDA-MB-231 cells transfected with microRNA negative control and miR-4306 mimic

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Gene array of human mammary epithelial cell HMEC transfected with miR-106a-3p mimic against transfected control mimic

Project description:Since a single miRNA can potentially target hundreds of genes. In parallel, to study the effect of the over-expression of miR-106a-3p on global gene expression patterns, we isolated total RNA from HMEC cells transfected with miR-106a-3p mimic and performed microarray analysis (HG-U133 Plus 2.0).

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Human Papillary thyroid cancer cell line, BCPAP control vs. miR-146b-5p mimic transfected

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RIP: miR-202 mimic-transfected HeLa cells vs. negative control

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