Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of Streptococcus pneumoniae during intraspecies competition in broth culture

ABSTRACT: RNA-seq analysis of Streptococcus pneumoniae during intraspecies competition in broth culture

PROVIDER: PRJNA434458 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6745290_1.fastq.gz	Fastqsanger.gz
	SRR6745290_2.fastq.gz	Fastqsanger.gz
	SRR6745291_1.fastq.gz	Fastqsanger.gz
	SRR6745291_2.fastq.gz	Fastqsanger.gz
	SRR6745292_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

RNA-seq analysis of Streptococcus pneumoniae during intraspecies competition in broth culture

Project description:Streptococcus pneumoniae (‘pneumococcus’) is a leading cause of morbidity and mortality worldwide and a frequent coloniser of the nasopharynx. Competition among bacterial members of the nasopharynx is believed to be mediated by bacteriocins: antimicrobial toxins produced by bacteria to inhibit growth of other bacteria. We have identified 14 newly-discovered bacteriocin gene clusters among pneumococci. Here, RNA-seq analysis was performed to investigate whether bacteriocin genes are transcriptionally active when two different strains are competing in broth culture. Pneumococcal reference strains PMEN3 (Spain9V-3) and PMEN6 (Hungary19A-6) were grown together in broth culture. Samples were taken at sequential time points and RNA was extracted and sequenced. The controls were prepared by growing each strain individually in identical experiential conditions and combining the RNA sequencing reads from each individual strain in silico. Differential expression analyses were performed by comparing reads generated when strains were competing in broth culture to those from when strains were grown individually.

2018-12-25 | GSE110750 | GEO

Changes in relative transcript amounts caused by heat in Streptococcus pneumoniae

Project description:RNA sequencing was used to explore the expression of all genes in Streptococcus pneumoniae under heat stress. The pneumococcal strain 2/2 was grown in broth culture under control (37°C) and high temperature (40°C) conditions. 2/2 culture samples were taken at sequential time points and RNA was extracted and sequenced.

2018-01-16 | GSE103778 | GEO

Changes in relative transcript amounts caused by the addition of mitomycin C in Streptococcus pneumoniae

Project description:Bacteriophages (phages) are widespread in Streptococcus pneumoniae, with most strains carrying phage genomes integrated into the chromosome. RNA sequencing was utilised to explore whether phage gene expression could be detected. The pneumococcal reference strain PMEN3 (Spain9V-3), which contained two full-length phages and one partial phage, was grown in broth culture and mitomycin C was added to facilitate phage induction. PMEN3 culture samples were taken at sequential time points and RNA was extracted and sequenced.

2017-02-20 | GSE89200 | GEO

CcpA Ensures Optimal Metabolic Fitness of Streptococcus pneumoniae

Project description:This SuperSeries is composed of the following subset Series: GSE31815: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Glucose at MID-log growth phase GSE31816: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + GLucose at transition-phase of growth (TS) GSE31817: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Galactose at MID-log growth phase GSE31818: ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + galactose at transition-phase of growth (TS) Refer to individual Series

2011-09-30 | E-GEOD-31819 | biostudies-arrayexpress

N-acetylgalactosamine Mediated Regulation of the aga Operon by AgaR in Streptococcus pneumoniae

Project description:Three Microarray comparisons have been preformed in this study. 1- Transcriptome comparison of the Streptococcus pneumoniae D39 wild type grown in M17 medium + 0.5 % (w/v) NAGa (NAGaM17) to M17 medium + 0.5 % (w/v) glucose (GM17) (GSM2372597 and GSM2372598). 2- Transcriptome comparison of the Streptococcus pneumoniae D39 ΔagaR to D39 wild type grown in M17 medium + 0.5 % (w/v) glucose (GM17) (GSM2372599 and GSM2372600). 3- Transcriptome comparison of the Streptococcus pneumoniae D39 ΔccpA to D39 wild type grown in M17 medium + 0.5 % (w/v) NAGa (NAGaM17) (GSM2290636 and GSM2290637).

2016-11-02 | GSE86008 | GEO

Bioplatforms Australia: Antibiotic Resistant Sepsis Pathogens Framework Initiative - Streptococcus pneumoniae

Project description:The Antibiotic Resistant Sepsis Pathogens Framework Initiative aims to develop a framework dataset of 5 sepsis pathogens (5 strains each) using an integrated application of genomic, transcriptomic, metabolomic and proteomic technologies. The pathogens included in this initiative are: Escherichia coli, Klebsiella pneumoniae complex, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. This submission pertains to Streptococcus pneumoniae strains 4496, 947, 4559, 180-2 and 180-15.

2021-07-05 | PXD017606 | Pride

Interactions with Commensal or Non-commensal Streptococci Alter Streptococcus mutans Transcriptome and Behaviors

Project description:RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. This revealed a common transcriptome pattern in S. mutans when grown in mixed-species culture, indepenedent of the species identity that S. mutans was cultured with. Additionally, transcriptome changes in the commensal species could also be determined when undergoing competition from S. mutans. RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture or in coculture with Streptococcus sobrinus NIDR 6715, Lactobacillus casei ATCC 4646 or Corynebacterium matruchotii ATCC 14266. These data were compared to previous coculture and quadculture RNA-Seq data with commensal streptococci (GSE209925). These data confirmed a common transcriptome pattern in S. mutans when grown in mixed-species culture with commensal streptococci that is not present with non-commensal streptococci, indepenedent of the species identity that S. mutans was cultured with.

2022-08-03 | GSE209925 | GEO

Transcriptional response of Streptococcus pneumoniae to copper

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM with 0mM to 0.05 mM copper.

2011-07-10 | GSE30413 | GEO

Bioplatforms Australia: Antibiotic Resistant Sepsis Pathogens Framework Initiative - Streptococcus pneumoniae 947

2021-07-01 | GSE152965 | GEO

Bioplatforms Australia: Antibiotic Resistant Sepsis Pathogens Framework Initiative - Streptococcus pneumoniae 4496

2021-07-01 | GSE152828 | GEO