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ABSTRACT: De novo transcriptome sequencing of Korean fir (Abies koreana)-2

PROVIDER: PRJNA435847 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR6781820_1.fastq.gz	Fastqsanger.gz
	SRR6781820_2.fastq.gz	Fastqsanger.gz
	SRR6781821_1.fastq.gz	Fastqsanger.gz
	SRR6781821_2.fastq.gz	Fastqsanger.gz
	SRR6781816_1.fastq.gz	Fastqsanger.gz

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Project description:De novo transcriptome sequencing of Korean fir (Abies koreana)

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Project description:The conservation of the endangered Korean fir, Abies koreana, is of critical ecological importance. In our previous study, a yeast-like fungus identified as Aureobasidium pullulans AK10, was isolated and shown to enhance drought tolerance in A. koreana seedlings. In this study, the effectiveness of A. pullulans AK10 treatment in enhancing drought tolerance in A. koreana was confirmed. Furthermore, using transcriptome analysis, we compared A. koreana seedlings treated with A. pullulans AK10 to untreated controls under drought conditions to elucidate the molecular responses involved in increased drought tolerance.

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Project description:We developed a transcriptome resource for Douglas-fir covering key developmental stages of megagametophytes over time: prefertilization, fertilization, embryogenesis, and early, unfertilized abortion. Extracted RNA was sequenced using large-scale sequencing and reads were assembled to generate a de novo reference transcriptome of 105,505 predicted high-confidence transcripts. Expression levels were estimated based on alignment of the original reads to the reference.

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Comparative transcriptome analyses of three medicinal Forsythia species and prediction of candidate genes involved in secondary metabolisms [Forsythia viridissima var. koreana]

Project description:Here, de novo transcriptome assemblies for leaf and flower tissues of Forsythias were performed, resulting in 81913 unigenes of F. suspensa, 88491 unigenes of F. viridissima and 69458 unigenes of F. koreana (F. viridissima var. koreana). Classification of the annotated unigenes by gene ontology terms and KEGG pathways was used to explore transcriptomic differences among the Forsythias. Orthogroup was introduced to compare expression levels of unigenes in tissues from different species, which unveiled that three leaf tissues of Fosythias were closely correlated based on expression values of orthologous unigenes. Showing high expression mainly in leaves of F. viridissima and F. koreana, candidate homologs for genes involved in the biosynthetic pathway of lignans and phenylethanoid glycosides were determined in these transcriptome assemblies.

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