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Transcriptome analysis of infected and uninfected mice with Citrobacter rodentium

ABSTRACT: Transcriptome analysis of infected and uninfected mice with Citrobacter rodentium

PROVIDER: PRJNA435929 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6782010.fastq.gz	Fastqsanger.gz
	SRR6782011.fastq.gz	Fastqsanger.gz
	SRR6782012.fastq.gz	Fastqsanger.gz
	SRR6782013.fastq.gz	Fastqsanger.gz
	SRR6782014.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of infected and uninfected mice with Citrobacter rodentium

Project description:Transcriptome analysis of infected and uninfected mice with Citrobacter rodentium

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Gene expression in IL-10-deficient and wild-type mice following Citrobacter rodentium infection

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Citrobacter rodentium triggers exaggerated mucosal responses in mice developing severe intestinal disease

Project description:Citrobacter rodentium is commonly used to elucidate mucosal responses to infection in mice developing mild disease (e.g. C57BL/6), while little is known about host responses to infection in mice developing severe disease (e.g. C3H/HeN). We report that the phyla Bacteroidetes is a minor component of the tissue-associated microbiome in uninfected C3H/HeN mice. Following infection, C. rodentium rapidly and uniformly colonises the C3H/HeN colonic mucosa, which coincides with downregulation of proteins involved in the TCA cycle and oxidative phosphorylation in intestinal epithelial cells (IECs). In contrast, we observed upregulation of DNA replication and DNA damage repair processes, as well as cholesterol biogenesis, import and export, nutritional immunity, IL-22 and INFg responses, and expression of NLRP3, in IECs. Moreover, C. rodentium triggers a staggered cell proliferation response from 3 days post infection, which correlated with a higher abundance of SLC5A9 and reduced abundance of the IEC differentiation markers SLC26A3 and CA4. Uniquely, C. rodentium triggers differential secretion of gel-forming mucins, with the number of goblet cells filled with acidic and neutral mucins dramatically increasing and decreasing, respectively. Together, these results show that despite vigorous responses, C3H/HeN mice succumb to C. rodentium infection, possibly as a result of excessive and disordered mucosal responses.

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