Project description:Edwardsiella tarda is a Gram-negative bacterial pathogen that can infect a wide range of freshwater and marine fish. However, the immune evasion mechanisms of Edwardsiella tarda is not fully understood. We found that Edwardsiella tarda infection generally significantly upregulated and downregulated a lot of immune-related genes of zebrafish ZF4 cells using RNA-seq technology.

2017-02-14 | GSE81773 | GEO

Gene expression profiles of vaccinated fish using a long-term efficiency mixed vaccine against Edwardsiella tarda performed by a new designed oligo DNA microarray in Japanese flounder Paralichthys olivaceus

Project description:Gene expression profiles by microarray have contributed for a elucidation of an immune-response and a determination of efficiency in vaccination. Recent day, edwardsielosis have caused a fatal damage in the aquaculture of Japanese flounder, Paralichthys olivaceus. However the formalin killed-cell vaccines made from Edwardsiella tarda isolated same fish species were not efficient. Recent our study revealed the mixed FKC vaccine made from the two different type of E. tarda protected Japanese flounder against Edwardsiella tarda infection for long-term. In this study, we analyzed the immune-response of a vaccinated fish kidney using the mixed FKC vaccine against Edwardsiella tarda with an Agilent custom-oligo DNA microarray on 9,573 probes of Japanese flounder. Our study revealed that the mixed FKC vaccine confered a strong immune-response and keeped a efficient for long-term on Japanese flounder.

2014-12-31 | GSE26808 | GEO

Modeling of the Response to a Multiple Vaccination Schedule Using the Adjuvant Neisseria meningitidis Porin B

Project description:Vaccine adjuvants enhance the immune response to vaccines, resulting more robust and durable immune responses. Although extensive work has been done on the response to a single vaccination over time, here we seek to characterize the response over the course of multiple vaccinations. In particular we modeled the changes in expression of mouse splenocytes after each of three vaccinations with and without antigen (chicken egg white ovalbumin) and adjuvant (N. meningitidis PorB). We also fitted the regulated genes into enriched gene sets to better characterize the response.

2018-09-26 | GSE104337 | GEO

Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection

Project description:This SuperSeries is composed of the following subset Series: GSE28481: Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection using a static immersion systems [experiment A] GSE28485: Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection [experiment B] Refer to individual Series

2011-09-30 | E-GEOD-28486 | biostudies-arrayexpress

RNAseq of skin vaccination array

Project description:The study aims to determine the molecular signature associated with varying applications of a novel skin vaccination array, compared to traditional needle and syringe immunization. The hypothesis is that the vaccination array induces a better immune response compared to the needle and syringe and this is due to a heightened inflammatory profile at the site of vaccination. We performed the study on wild-type mice that received varying forms of immunization, including intradermal needle and syringe with or without vaccine, Nanopatch with or without vaccine, Nanopatch with vaccine with QS-21 adjuvant, and Nanopatch with vaccine at higher application energy.

2019-11-20 | E-MTAB-7482 | biostudies-arrayexpress

Propioniciclava tarda

Project description:Propioniciclava tarda overview

| PRJNA368772 | ENA

Otis tarda

Project description:Otis tarda overview

| PRJNA903976 | ENA