Project description:Comparison of temporal small RNA expression levels in skin between different human age cohorts. RNA-seq data comprise 4 age groups: 24-29 years, 45-50 years, 60-65years and 75-80 years. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Comparison of temporal small RNA expression levels in blood between different human age cohorts. RNA-seq data comprise 4 age groups: 24-29 years, 45-50 years, 60-65 years and 75-80 years. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Changes in the transcriptomes of human tissues with advancing age are poorly cataloged. Here, we set to identify the coding and long noncoding RNAs present in cultured primary skin fibroblasts collected from 82 healthy individuals across a wide age spectrum (22 to 89 years old) who participated in the GESTALT (Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing) study of the National Institute on Aging, NIH. Using high-throughput RNA sequencing and a linear regression model, we identified 1,437 coding RNA (mRNA) and 1,177 linear and circular long noncoding (lncRNA) that were differentially abundant as a function of age. GSEA analysis revealed select transcription factors implicated in coordinating the transcription of subsets of differentially abundant mRNAs, while LncSEA identified RNA-binding proteins predicted to contribute to the age-associated lncRNA profiles. In sum, we report age-associated changes in the global transcriptome, coding and noncoding, from healthy human skin fibroblasts, and propose that they might serve as biomarkers and therapeutic targets in aging skin.
Project description:Comparison of gene expression profiles from Mus musculus skin of two age groups. The RNA-seq data comprise 2 groups at ages: 2 and 9 months. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Comparison of gene expression profiles from Nothobranchius furzeri (strain: GRZ) skin of different age groups. The RNA-seq data comprise 4 groups: 5, 7, 10, and 14 weeks. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Background: Comparison of temporal gene expression profiles. The RNA-seq data comprises 3 age groups: 2, 15 and 30 months for mouse skin; 5, 24 and 42 months for zebrafish skin. Illumina 50bp single-stranded single-read RNA sequencing Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)