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Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation

ABSTRACT: Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation

PROVIDER: PRJNA436649 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6798160_1.fastq.gz	Fastqsanger.gz
	SRR6798160_2.fastq.gz	Fastqsanger.gz
	SRR6798170_1.fastq.gz	Fastqsanger.gz
	SRR6798170_2.fastq.gz	Fastqsanger.gz
	SRR6798161_1.fastq.gz	Fastqsanger.gz

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Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation [histone marks]

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Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation [ChIP-seq]

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Systematic Perturbation of Thousands of Retroviral LTRs in Mouse Embryos

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Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation [RNA-seq]

Project description:Recent work suggests extensive adaptation of transposable elements (TEs) for host gene regulation. However, high numbers of integrations typical of TEs, coupled with sequence divergence within families, have made systematic interrogation of the regulatory contributions of TEs challenging. Here, we employ CARGO, our recent method for CRISPR gRNA multiplexing, to facilitate targeting of LTR5HS, a higher primate-specific class of HERVK (HML-2) LTRs that is active during early development and present in ~700 copies throughout the human genome. We combine CARGO with CRISPR activation or interference to, respectively, induce or silence LTR5HS en masse, and demonstrate that this system robustly targets the vast majority of LTR5HS insertions. Remarkably, activation/silencing of LTR5HS is associated with reciprocal up- and down-regulation of hundreds of human genes. These effects require presence of retroviral sequences, but occur over long genomic distances, consistent with a pervasive function of LTR5HS elements as early embryonic enhancers in higher primates.

2018-08-16 | GSE111332 | GEO

Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation [ChIP-seq]

2018-08-16 | GSE111331 | GEO

Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation [histone marks]

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Systematic Perturbation of Thousands of Retroviral LTRs in Mouse Embryos [ChIP-Seq]

Project description:Systematic Perturbation of Thousands of Retroviral LTRs in Mouse Embryos [ChIP-Seq]

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Systematic Perturbation of Thousands of Retroviral LTRs in Mouse Embryos [RNA-Seq]

Project description:Systematic Perturbation of Thousands of Retroviral LTRs in Mouse Embryos [RNA-Seq]

| PRJNA1011393 | ENA

Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation.

Project description:Recent work suggests extensive adaptation of transposable elements (TEs) for host gene regulation. However, high numbers of integrations typical of TEs, coupled with sequence divergence within families, have made systematic interrogation of the regulatory contributions of TEs challenging. Here, we employ CARGO, our recent method for CRISPR gRNA multiplexing, to facilitate targeting of LTR5HS, an ape-specific class of HERVK (HML-2) LTRs that is active during early development and present in ~700 copies throughout the human genome. We combine CARGO with CRISPR activation or interference to, respectively, induce or silence LTR5HS en masse, and demonstrate that this system robustly targets the vast majority of LTR5HS insertions. Remarkably, activation/silencing of LTR5HS is associated with reciprocal up- and down-regulation of hundreds of human genes. These effects require the presence of retroviral sequences, but occur over long genomic distances, consistent with a pervasive function of LTR5HS elements as early embryonic enhancers in apes.

| S-EPMC6158008 | biostudies-literature

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