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RNA-sequencing of Adult Mouse Pillar and Deiters’ Supporting Cells of the Organ of Corti

ABSTRACT: RNA-sequencing of Adult Mouse Pillar and Deiters’ Supporting Cells of the Organ of Corti

PROVIDER: PRJNA436759 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6798460_1.fastq.gz	Fastqsanger.gz
	SRR6798460_2.fastq.gz	Fastqsanger.gz
	SRR6798470_1.fastq.gz	Fastqsanger.gz
	SRR6798470_2.fastq.gz	Fastqsanger.gz
	SRR6798461_1.fastq.gz	Fastqsanger.gz

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RNA-sequencing of Adult Mouse Pillar and Deiters’ Supporting Cells of the Organ of Corti

Project description:To understand the basic biological properties of supporting cells of the mammalian inner ear, we examined the transcriptome of adult pillar and Deiters’ cells. Morphologically distinct pillar and Deiters’ cells were isolated from the organ of Corti from adult CBA/J mice. One thousand pillar and Deiters’ cells were separately collected for each biological replicate, using the suction pipette technique. RNA sequencing of three biological replicates, each with two technical repeats, was performed. The resulting sequenced reads were mapped. Comparisons between pillar cells and Deiters’ cells allow identification of enriched genes, as well as differentially expressed genes that result in cellular specialization. Our dataset provides an extensive resource for understanding the molecular mechanisms underlying morphology, function, and pathology of adult mouse pillar and Deiters’ cells.

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Epigenetic analysis of perinatal maturation of supporting cells in the organ of Corti

Project description:Sensory hair cells cannot be regenerated through transdifferentiation of neighboring supporting cells in the organ of Corti in mature mammalian animals, but limited regeneration capacity exists in supporting cells at neonatal stage in mouse and this transdifferentiation potential is rapidly lost during the first week of postnatal maturtion. We hypothesized that epigenetic decommissioning of hair cell gene enhancers in supporting cells during postnatal maturation leads the permanent silencing of hair cell genes and the loss of transdifferentiation potential. To test this hypothesis, we FACS purified hair cells and supporting cells from cochleae at different developmental stages for transcritomic analysis (RNAseq), chromatin accessibility assay (ATACseq) and histone modification profiling (ChIPseq or CUT&RUN). We first defined hair cell genes and predicted their potential active enhancers. We found that hair cell gene promoters and enhancers were kept in a primed-but-silenced status (H3K4me1/3+, low H3K27ac but high H3K27me3) in supporting cells at neonatal stage. During postnatal maturation, hair cell gene enhancers are decommissioned through H3K4me1 removal, leading to the permanent silencing of hair cells genes. We also found that hair cell gene enhancer decommissioning process correlated with the base-to-apex wave of transdifferentiation potential loss. In addition, hair cell gene enhancer commissioning status is preserved in mature utricular supporting cells, which can regenerate hair cells through transdifferentiation even at adult stage. Those data together suggest that decommissioning of hair cell gene enhancers in supporting cells during postnatal maturation is the epigenetic mechanism underlying the loss of regeneration capacity in the organ of Corti.

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RNA-seq of Adult Mouse Pillar and Deiters Supporting Cells, Inner and Outer Hair Cells of the Organ of Corti

Project description:RNA-seq of Adult Mouse Pillar and Deiters Supporting Cells, Inner and Outer Hair Cells of the Organ of Corti

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Epigenetic analysis of perinatal maturation of supporting cells in the organ of Corti

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Project description:To further understand the biological properties of hair cells of the mammalian cochlea, we examined the transcriptome of adult inner and outer hair cells. Morphologically distinct inner and outer hair cells were isolated from the organ of Corti from adult CBA/J mice. One thousand inner and outer hair cells were separately collected for each biological replicate, using the suction pipette technique. RNA sequencing of two biological replicates of IHCs and three biological replicates of OHCs, each with two technical repeats, was performed. The resulting sequenced reads were mapped. Comparisons between inner and outer hair cells allow identification of enriched genes, as well as differentially expressed genes that result in cellular specialization. Our dataset provides an extensive resource for understanding the molecular mechanisms underlying morphology, function, and pathology of adult mouse inner and outer hair cells.

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RNA-sequencing of Adult Mouse Inner Hair Cells and Outer Hair Cells of the organ of Corti

Project description:RNA-sequencing of Adult Mouse Inner Hair Cells and Outer Hair Cells of the organ of Corti

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Transcripts identified by microarray analysis that were deregulated in E18.5 Sox2(creERT2);Tbx2(fl/fl) (Sox2-Tbx2LOF) organ of Corti.

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