Project description:To identify breast cancer metastasis-relevant circRNAs, we assessed the expression profiles of circRNAs in parental MDA-MB-231 (231-PAR) cells, isogenic brain metastatic cells (231-BM6), lung metastatic cells (LM2) and bone metastatic cells (1833), which were isolated from brain, lung or bone-seeking 231-PAR cells
Project description:Circular RNAs (circRNAs) are key regulators in the development and progression of human cancers. A human circRNA microarray analysis was performed to screen for abnormally expressed circRNAs in breast cancer tissue and circRNA-000911 was identified as a circRNA which was significantly downregulated in breast cancer cells. Mechanistic investigations suggested that the enhanced expression of circRNA-000911 suppressed cell proliferation, migration and invasion, and promoted the apoptosis of breast cancer cells. The overexpression of circRNA-000911 may provide a future direction which may aid in the development of a novel treatment strategy for breast cancer.
Project description:Brain metastasis is a significant complication of breast cancer, and its molecular determinants vary across breast cancer subtypes. This study examined transcriptomic differences associated with brain metastasis using RNA sequencing of breast cancer tissues. The deposited RNA-seq dataset contains 42 analyzed biological samples, comprising 23 samples classified as brain-metastasis positive and 19 as brain-metastasis negative. Differential gene-expression and functional enrichment analyses were used to characterize brain-metastasis-associated transcriptional programs across Luminal A, Luminal B, HER2-enriched, and triple-negative breast cancer subtypes.
Project description:circRNA microArray analysis from Arraystar were used to examine the expression profile in HCC with metastasis, HCC without metastasis, and non-tumor tissues