Project description:Outputs from single-cell RNA-seq sequenced reads from isolated mouse epithelial cells from homeostatic submucosal glands and Naphthalene-injured surface epithelium of adult mouse airway (P70)
Project description:We report the application of single-cell-based sequencing technology for high-throughput profiling of cell types and and transcriptional state of cells in the complex tissue of the human airway epithelium. Our model system is that of polarized human airway epithelial cultures, differentiated from hTert-immortalized basal-like precursor cells.
Project description:To characterize the host response to pandemic IAV infection in its natural target cells, we infected primary human airway epithelial cell (hAEC) cultures wild-type pandemic IAV (WT) or a NS1 mutant virus (NS1R38A) with abrogated dsRNA binding capacity at a multiplicity of infection (MOI) of 0.03. We then profiled the transcriptomes of uninfected cells as well as cells harvested 18 hours post-infection (hpi) using single-cell RNA sequencing (scRNA-seq).
Project description:We conducted the first single-cell RNA sequencing (scRNA-seq) analysis of Tollip knockdown (TKD) human airway epithelial cells to determine the cell-type-specific role of TKD and its interaction with IL-13.
Project description:A study employs quantitative phosphoproteomics approch for systems biology understanding of epithelial mesenchymal transition of human primary small airway epithelial cells
Project description:A study employs quantitative phosphoproteomics approach fro systems biology understanding of the epithelial mesenchymal transition of human primary small airway epithelial cells.
Project description:A study employs quantitative proteomics approach fro systems biology understanding of the epithelial mesenchymal transition of human primary small airway epithelial cells.