Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA 5-methylcytosine in Paramecium

ABSTRACT: DNA 5-methylcytosine in Paramecium

PROVIDER: PRJNA437583 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6820760_1.fastq.gz	Fastqsanger.gz
	SRR6820760_2.fastq.gz	Fastqsanger.gz
	SRR6820758_1.fastq.gz	Fastqsanger.gz
	SRR6820758_2.fastq.gz	Fastqsanger.gz
	SRR6820759_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

DNA 5-methylcytosine in Paramecium

Project description:5-methyl-cytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. In this study, we applied bisfulfite genome sequencing, DNA mass spectrometry and antibody-based fluorescence detection to investigate the presence of DNA methylation in Paramecium tetraurelia. While the antibody-based methods suggest cytosine methylation, DNA mass spectrometry and bisulfite sequencing reveal that levels are actually below the limit of detection. Our results suggest that Paramecium does not utilize 5-methyl-cytosine DNA methylation as an integral part of its epigenetic arsenal.

2018-04-01 | GSE111621 | GEO

Paramecium tetraurelia reciliation (stationary phase)

2009-11-01 | GSE14631 | GEO

Paramecium

Project description:Paramecium Mitochondrial Variation

| PRJNA490059 | ENA

Paramecium subcellular spatial proteomics

Project description:To gain insight into how Paramecium cells reorganize their subcellular structure during endosymbiosis stage, we generated a comprehensive spatial proteomics map across aposymbiotic Paramecium and endosymbiotic Paramecium

2024-10-18 | PXD055842 | Pride

Paramecium tetraurelia reciliation (log phase)

Project description:Paramecium cells in log-phase growth were treated for deciliation and total mRNA extracted at two time points after deciliation. This is usefull to study genes involved in cilia biogenesis. Time points are : T0 : mRNA extracted before deciliation. T30 : mRNA extracted 30 minutes after deciliation. T120 : mRNA extracted 120 minutes after deciliation.

2009-11-01 | GSE12620 | GEO

DNA 5-methylcytosine specific profiling with limited input

Project description:DNA 5-methylcytosine specific profiling with limited input

| PRJNA449368 | ENA

Paramecium tetraurelia trichocyst exocytosis recovery

Project description:Paramecium cells possess a few thousand trichocysts (big secretory granules of 3~4 um long) docked at their surface and whose discharge in the external medium can be triggered using aminoethyldextran (a modified dextran positively charged) without damage to the cells. After discharge, new trichocysts are re-synthesized by the cells. We studied the evolution of the transcriptome during this synthesis.

2010-07-07 | GSE17930 | GEO

Transcriptome analysis, Paramecium tetraurelia ICL (as a control) vs. Rdr3 silencing

Project description:Differential transcriptome of Paramecium tetraurelia strain 51 undergoing RNAi by feeding against ICL7a (as a control) and RDR3 for nine days.

2014-07-15 | GSE59390 | GEO

Structural maintenance of chromosomes (SMCs) proteins are required for developmental DNA elimination in Paramecium tetraurelia

Project description:To study the role of structural maintenance of chromosome (SMC)4-1 and SMC4-2 during programmed genome rearrangement in Paramecium tetraurelia. Paramecium SMC4-1 and SMC4-2 was tagged with 3 FLAG and HA at its C-terminal separately. The recombinant plasmid was microinjected into macronuclear and used for co-immunoprecipitation and Mass spectrometry studies to identify interacting proteins of SMC4-1 and SMC4-2 that indicates the different functions between Paramecium SMC4s.

2023-12-05 | PXD041826 | Pride

Paramecium tetraurelia reciliation (stationary phase)

Project description:Paramecium cells in stationary phase were treated for deciliation and total mRNA extracted at two time points (45 and 130 minutes) after deciliation. Keywords: Time course analysis of expression during reciliation Experimental design has two biological replications for the 45 and 130 minutes time points and only one sample for the reference (T0) time point.

2010-06-20 | E-GEOD-14631 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data