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UXT is required for spermatogenesis in mice

ABSTRACT: UXT is required for spermatogenesis in mice

PROVIDER: PRJNA438005 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6828160.fastq.gz	Fastqsanger.gz
	SRR6828161.fastq.gz	Fastqsanger.gz
	SRR6828162.fastq.gz	Fastqsanger.gz
	SRR6828163.fastq.gz	Fastqsanger.gz
	SRR6828164.fastq.gz	Fastqsanger.gz

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UXT is required for spermatogenesis in mice

Project description:Male mammals must simultaneously produce prodigious numbers of sperm and maintain an adequate reserve of stem cells to ensure continuous production of gametes throughout life. Failures in the mechanisms responsible for balancing germ cell differentiation and spermatogonial stem cell (SSC) self-renewal can result in infertility. We discovered a novel requirement for Ubiquitous Expressed Transcript (UXT) in spermatogenesis by developing the first knockout mouse model for this gene. Constitutive deletion of Uxt is embryonic lethal, while conditional knockout in the male germline results in a Sertoli cell-only phenotype during the first wave of spermatogenesis that does not recover in the adult. This phenotype begins to manifest between 6 and 7 days post-partum, just before meiotic entry. Gene expression analysis revealed that Uxt deletion downregulates the transcription of genes governing SSC self-renewal, differentiation, and meiosis, consistent with its previously defined role as a transcriptional co-factor. Our study has revealed the first in vivo function for UXT in the mammalian germline as a regulator of distinct transcriptional programs in SSCs and differentiating spermatogonia.

2018-04-18 | GSE111740 | GEO

SRSF2 is required for mRNA splicing and spermatogenesis.

Project description:SRSF2 is required for mRNA splicing and spermatogenesis.

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Proper chromatin programming during spermatogenesis is required for embryonic development

Project description:Epigenetic mechanisms including DNA methylation, non-coding RNAs and histone modifications control gene expression. Studies suggest that a father's lifetime experiences can be transmitted to his offspring to affect development and health. The mechanisms underlying such epigenetic inheritance are unknown. A potential route for paternal transmission is the unique chromatin composition of spermatozoa. Unlike somatic cells and oocytes, most nucleosomes in sperm are replaced with protamine nucleoproteins. The role of residual nucleosomes, residing at gene regulatory sequences, for epigenetic control of embryonic development is unknown. Here we generated a transgenic mouse model in which over-expression of the histone H3 lysine 4 (H3K4) demethylase LSD1/KDM1A during spermatogenesis alters H3K4 methylation in sperm. Strikingly, KDM1A over-expression in one generation causes severe embryonic defects in non-transgenic descendants spanning three subsequent generations. We show for the first time that correct histone methylation homeostasis during spermatogenesis is critical for offspring development and survival over multiple generations. Identification of H3K4me2 and nucleosome occupancies in sperm of wildtype mice, KDM1A transgenic mice and their non-transgenic littermates.

2015-11-03 | E-GEOD-55471 | biostudies-arrayexpress

SRSF2 is required for mRNA splicing and spermatogenesis [LACE-seq]

Project description:SRSF2 is required for mRNA splicing and spermatogenesis [LACE-seq]

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SRSF2 is required for mRNA splicing and spermatogenesis [RNA-seq]

Project description:SRSF2 is required for mRNA splicing and spermatogenesis [RNA-seq]

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DNA/RNA helicase DHX36 is required for late stages of spermatogenesis

Project description:Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, play crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we delete Dhx36 in advanced germ cells with Stra8-GFPCre, and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 play crucial roles in the late stages of spermatogenesis.

2022-11-24 | GSE218477 | GEO

Transcriptome changes after overexpression, knockdown and condItional knockout of UXT in mice

Project description:Ubiquitously expressed transcript (UXT) has been shown to play a role in regulating the process of autophagy. Here, we investigated roles of UXT in the innnate immune responses after overexpression, knockdown and condItional knockout of UXT in mice

2024-05-18 | GSE175928 | GEO

DNA/RNA helicase DHX36 is required for late stages of spermatogenesis

Project description:DNA/RNA helicase DHX36 is required for late stages of spermatogenesis

| PRJNA903907 | ENA

NAT10-mediated N4-Acetylcytidine is Required for Spermatogenesis and Male Fertility

Project description:NAT10-mediated N4-Acetylcytidine is Required for Spermatogenesis and Male Fertility

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Project description:CEP128 Mutations Impair Spermatogenesis in Humans and Mice

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