Project description:We sequenced and analyzed the genome of a highly inbred miniature Chinese pig strain, the Banna Minipig Inbred Line (BMI). we conducted whole genome screening using next generation sequencing (NGS) technology and performed SNP calling using Sus Scrofa genome assembly Sscrofa11.1.
Project description:Successful production of offspring in mammals is determined by the growth and apoptosis pathway, which is responsible for maintaining the balance between the estrous cycle. It is also believed that the development of the porcine ovary is regulated similarly; however, the molecular mechanism underlying differences in follicle development in the minipig and pig has yet to be elucidated. The present study aimed to identify developmental-associated genes differentially expressed in the minipig vs. pig.
Project description:Here, we performed the first whole-genome bisulfite sequencing (WGBS) of longissimus dorsi muscle (LDM) from Landrace (LR) and Wuzhishan (WZS) miniature pig during early embryonic stages (18, 21 and 28 dpc (days post coitum)).
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples