Project description:We detected the expression of miRNA in a fibrosarcoma with ESCC sample and the corresponding normal esophagus tissue. Then, we identified the differentially expressed miRNAs in this rare disease.
Project description:We detected DNA methylation of a fibrosarcoma with ESCC sample and the corresponding normal sample to identify the aberrant DNA methylation status in this rare disease.
Project description:We detected the expression of miRNA in 15 ESCC samples' tumor tissues and the corresponding normal esophagus tissue. Then, we identified the differentially expressed miRNAs in ESCC.
Project description:To understand the difference of protein expression between paired esophageal squamous cell carcinoma (ESCC) and adjacent normal tissues, we collected 10 paired ESCC and normal tissues from surgical resected specimems for high-throughput proteomic experiments. From comparative analysis, the dysregulated signaling pathways in ESCC could be uncovered.
Project description:To discover ESCC related proteins, we used SWATH to quantify the protein abundance between ESCC and adjacent tissues. Briefly, we pooled 10 ESCCtissues and their corresponding adjacent tissues for SWATH acquisition with three replicates.Three DDA repeats were also acquired with the pooled 10-paired ESCC tissue.The trypsin digested peptide mixture was analyzed by AB SCIEX 5600 (AB SCIEX).The database searching procedure was achieved using ProteinPilot v4.5 (AB Sciex). The database is IPI_homo_sapiens_V3.87.