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Novel RNA partners of the Mei2 RNA binding protein during early meiosis in Schizosaccharomyces pombe

ABSTRACT: Novel RNA partners of the Mei2 RNA binding protein during early meiosis in Schizosaccharomyces pombe

PROVIDER: PRJNA439321 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6872120_1.fastq.gz	Fastqsanger.gz
	SRR6872120_2.fastq.gz	Fastqsanger.gz
	SRR6872116_1.fastq.gz	Fastqsanger.gz
	SRR6872116_2.fastq.gz	Fastqsanger.gz
	SRR6872118_1.fastq.gz	Fastqsanger.gz

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Novel RNA partners of the Mei2 RNA binding protein during early meiosis in Schizosaccharomyces pombe

Project description:Novel RNA partners of the Mei2 RNA binding protein during early meiosis in Schizosaccharomyces pombe

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Project description:Meiosis is a specialized cell division that generates gametes, such as eggs and sperm. Errors in meiosis result in miscarriages and are the leading cause of birth defects, however the molecular origins of these defects remain unknown. Studies in model organisms are beginning to identify the genes and pathways important for meiosis, but the parts list is still poorly defined. Here we present a comprehensive catalogue of genes required for meiosis in the fission yeast, Schizosaccharomyces pombe. Our genome-wide functional screen surveyed all non-essential genes for roles in chromosome segregation and spore formation. Novel genes required at distinct stages of the meiotic chromosome segregation and differentiation programme were identified. Preliminary characterization implicated three of these genes in centrosome/spindle pole body function, centromere and cohesion function. Our findings represent a near-complete parts list of genes required for meiosis in fission yeast, providing a valuable resource to advance our molecular understanding of meiosis.

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Project description:We used RIP-CHIP to determine the RNAs bound specifically to RNA binding protein Mei2 in early meiosis in fission yeast.

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Novel RNA partners of the Mei2 RNA binding protein during early meiosis in Schizosaccharomyces pombe

Project description:We used CLIP-Seq to determine the RNAs bound specifically to RNA binding protein Mei2 in early meiosis in fission yeast. We added a TAP tag to the C-terminal ends of two meiotic RNA binding proteins, Mei2 and Msa1. We used an untagged fission yeast strain as a negative control. These strains were nitrogen starved and allowed to progress into meiosis, after which they were harvested, lyzed and crosslinking immunoprecipitaion was performed. The RNAs purified after CLIP were sequenced

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