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Cold priming and triggering of Arabidopsis accessions

ABSTRACT: Cold priming and triggering of Arabidopsis accessions

PROVIDER: PRJNA445300 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6880730.fastq.gz	Fastqsanger.gz
	SRR6880740.fastq.gz	Fastqsanger.gz
	SRR6880750.fastq.gz	Fastqsanger.gz
	SRR6880731.fastq.gz	Fastqsanger.gz
	SRR6880741.fastq.gz	Fastqsanger.gz

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Cold priming and triggering of Arabidopsis accessions

Project description:Plants from temperate regions can be primed by exposure to low, non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold priming has been investigated in detail, hardly anything is known about memory of a previous cold event under warm conditions and a following low temperature triggering event. We show that three days of cold priming at 4°C, a seven-day lag phase at 20°C and a triggering treatment of 4°C improved the freezing tolerance of Arabidopsis Col-0 and other accessions compared to plants that were not primed before. Transcripts, metabolites and lipids as possible molecular determinants of this increase in freezing tolerance were investigated in Arabidopsis accessions Col-0 and N14 after priming, memory phase and triggering by Illumina-based RNA-Seq, GC-MS metabolite profiling and UPLC FT-MS-based lipidomics. Comparing primed and triggered with only triggered samples 93 and 128 unique differentially expressed genes could be identified in Col-0 and N14, together with three and six significantly changed lipids and one metabolite in N14. Possible functions of these candidates will be discussed. This work identified for the first time molecular and metabolic changes accompanying cold stress memory and triggering by a second cold stress.

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Specific CBF transcription factors and cold‐responsive genes fine‐tune the early triggering response after acquisition of cold priming and memory

Project description:Plants need to adapt to fluctuating temperatures throughout their lifetime. Previous research has shown that A. thaliana retains memory of a first cold stress (priming) and improves its primed freezing tolerance even further when subjected to a second similar stress after a lag phase. This study investigates primary metabolomic (gas chromatography–mass spectrometry) and transcriptomic (RNA-Seq) changes during 24 h of cold priming or cold triggering at 4°C. During triggering higher expression of genes encoding Late Embryogenesis Abundant (LEA), antifreeze proteins or proteins function as detoxifiers of reactive oxygen species (ROS) was observed compared to cold priming. Examples of early responders to triggering were xyloglucan endotransglucosylase/hydrolase genes encoding proteins involved in cell wall remodeling while late responders were identified to act in fine-tuning of stress response and development regulation. Four transcription factors, CBF2/DREB1C, CBF4/DREB1D, DDF2/DREB1E and DDF1/DREB1F were strongly and uniquely significantly induced throughout the entire triggering response. The induction of unusual members of the DREB subfamily of ERF/AP2 transcription factors, the relatively small number of induced genes of the CBF regulon and slower accumulation of selected cold stress associated metabolites proposes that a cold triggering stimulus might be sensed as milder stress in plants compared to priming. Further, the strong induction of CBF4 throughout triggering suggests a unique function of this gene during cold stress memory.

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Project description:We analysed the effect of cold priming on cold and high light regulation of gene expression. 5 days after the first cold treatment the primary stress response was widely reset. Then, a second (triggering) cold stimulus (24 h 4 °C) and a triggering high-light stimulus (2 h 800 µmol quanta m-2 s-1), which regulate many stress responsive genes in the same direction in naïve plants, caused widely specific and even inverse regulation of priming-responsive genes.

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