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Genetic characterization of macrophages from induced sputum of asthma and COPD patients

ABSTRACT: Genetic characterization of macrophages from induced sputum of asthma and COPD patients

PROVIDER: PRJNA445404 | ENA |

REPOSITORIES: ENA

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Genetic characterization of macrophages from induced sputum of asthma and COPD patients

Project description:Background: Macrophages are important cells in pathogenesis of obstructive lung diseases including asthma and chronic obstructive pulmonary disease (COPD). The aim of the study was a multivariate, genetic, comparative analysis of macrophages from patients with asthma and COPD. Methods: Macrophages were isolated from induced sputum (IS) by magnetic bead separation. Transcriptomic measurements were carried out using Affymetrix Human Gene 2.1 ST ArrayStrip in 17 samples: 8 asthma (4 ICS naive , 4 ICS treated), 4 COPD and 5 control samples. The expression of the most significantly differentiating genes was evaluated by real time PCR in 34 samples (15 patients with asthma, 11 patients with COPD and 17 controls). Results: USP53 was the only gene differentially expressed (p adjusted clue =0.09) in the comparison between ICS naïve and ICS treated asthma. The PCR verification showed increased expression of BTF3, CDS2, DNAJC13, DDX5, GNAI2, SCGB1A1, SIRPB1, TRAF3IP, USP53, WDR49 in the asthma compared to COPD group. The changed gene expression profile of macrophages were characterized mostly by gene ontology terms linked with cell motility, cilium function, cell junction and adhesion organization. Conclusions: Gene expression profiling of sputum macrophages revealed distinct molecular capacity in asthma and COPD. The role of sputum macrophages in the pathophysiology of obstructive lung diseases is probably connected with biological processes associated with their motility, cilium dysfunction and cell junction organization.

2018-08-09 | GSE112260 | GEO

Analysis of sputum methylation data in Asthma and COPD

Project description:We evaluated the applicability and usability of whole-genome methylomics of sputum samples in molecular profiling of chronic inflammatory lung diseases. Genomic DNA was purified from sputum samples of subjects with Asthma, COPD as well as healthy controls and analyzed on the Illumina Infinium HumanMethylation 450k platform.

2020-10-25 | GSE148000 | GEO

Analysis of sputum gene expression data in Asthma and COPD

Project description:In addition to analyzing whole-genome methylation, we concomitantly evaluated sputum cell gene expression in the context of chronic inflammatory lung disease. Nucleic acids were purified from sputum samples of subjects with Asthma, COPD as well as healthy controls. Gene expression was analyzed on the Agilent Human GE 4x44k v2 platform.

2020-10-25 | GSE148004 | GEO

Analysis of sputum methylation data in Asthma and COPD

Project description:Analysis of sputum methylation data in Asthma and COPD

| PRJNA622793 | ENA

Analysis of sputum gene expression data in Asthma and COPD

Project description:Analysis of sputum gene expression data in Asthma and COPD

| PRJNA622797 | ENA

Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Project description:Induced sputum is used to sample inflammatory cells, predominantly neutrophils and macrophages, from the airways of COPD patients. Our aim was to identify candidate genes associated with the degree of airflow obstruction and the extent of emphysema by expression profiling, and then to confirm these findings for selected candidates using specific PCR and protein analysis. Two sputum studies were performed in GOLD stage 2 -4 COPD ex-smokers from the ECLIPSE cohort. First, gene array profiling at baseline in 1480 patients was performed. At year 1, samples from a separate population of 176 patients were used for real-time PCR. The gene expression findings for IL-18R were further analysed using immunohistochemistry in lung tissue and induced sputum samples from patients outside the ECLIPSE cohort.

2010-06-16 | E-GEOD-22148 | biostudies-arrayexpress

Transcriptomic analysis of induced sputum samples in severe asthma

Project description:Gene expression profiles were generated from induced sputum samples in asthma and healthy controls. The study identified differential gene expression and pathways in severe asthma.

2019-09-12 | GSE137268 | GEO

Proteomic analysis of human eosinophils in patients with asthma-COPD overlap and eosinophilic COPD

Project description:Activated eosinophils is a major cell type to be mainly involved in allergic diseases. Recent studies also indicated that eosinophils play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD), especially asthma-COPD overlap and/or eosinophil COPD. The aim of this study is to clarify cellular characters of human eosinophils in patients with asthma-COPD overlap and/or eosinophil COPD.

2024-06-20 | PXD052550 | JPOST Repository

Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

2010-06-08 | GSE22148 | GEO

A sputum gene expression signature of six biomarkers identifies asthma inflammatory phenotype and steroid responsiveness

Project description:This study identifies differentially expression genes in the sputum of people with eosinophilic, neutrophilic and paucigranulocytic asthma. A selection of markers identified using this microarray were further validated using qPCR on a wider sample set. Gene expression profiles were generated from induced sputum samples from 47 asthma patients and were grouped by the inflammatory phenotype assigned using sputum cell counts into neutrophilic asthma (n=12), eosinophilic asthma (n=17) and paucigranulocytic asthma (n=18). RNA was extracted, amplified and hybridised to Illumina Sentrix HumanRef-8 Version 2 Expression BeadChips, and genes that were differentially expressed between asthma inflammatory phenotypes were compared.

2014-03-25 | E-GEOD-45111 | biostudies-arrayexpress

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