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ABSTRACT: Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR

PROVIDER: PRJNA445514 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6897060.fastq.gz	Fastqsanger.gz
	SRR6897070.fastq.gz	Fastqsanger.gz
	SRR6897080.fastq.gz	Fastqsanger.gz
	SRR6897090.fastq.gz	Fastqsanger.gz
	SRR6897100.fastq.gz	Fastqsanger.gz

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: Lung epithelia cells were collected after 10 days of treatment by pseudomonas aeruginosa (PA), RNA-Seq and qRT-PCR were applied to analyze the high-throughput data Results: In general, the mutant type II-like cells expressed higher levels of type I cell markers and lower levels of type II cell markers

2019-03-12 | GSE124259 | GEO

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